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Titolo:
VOLTAGE-ACTIVATED CALCIUM-CHANNEL CURRENTS OF RAT DRG NEURONS ARE REDUCED BY MERCURIC-CHLORIDE (HGCL2) AND METHYLMERCURY (CH3HGCL)
Autore:
LEONHARDT R; PEKEL M; PLATT B; HAAS HL; BUSSELBERG D;
Indirizzi:
UNIV DUSSELDORF,INST PHYSIOL 2,HUFELANDST 55 D-45122 DUSSELDORF GERMANY UNIV DUSSELDORF,INST PHYSIOL 2 D-45122 DUSSELDORF GERMANY
Titolo Testata:
Neurotoxicology
fascicolo: 1, volume: 17, anno: 1996,
pagine: 85 - 91
SICI:
0161-813X(1996)17:1<85:VCCORD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT GANGLION-CELLS; MODULATION; CULTURE;
Keywords:
MERCURIC CHLORIDE (HGCL2); METHYLMERCURY (CH3HGCL); CALCIUM CHANNEL CURRENTS; RAT DRG NEURONS; WHOLE CELL PATCH CLAMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
R. Leonhardt et al., "VOLTAGE-ACTIVATED CALCIUM-CHANNEL CURRENTS OF RAT DRG NEURONS ARE REDUCED BY MERCURIC-CHLORIDE (HGCL2) AND METHYLMERCURY (CH3HGCL)", Neurotoxicology, 17(1), 1996, pp. 85-91

Abstract

The actions of bath applied mercuric chloride (HgCl2) and methylmercury (CH3HgCl) on voltage-activated calcium channel currents (VACCCs) were tested, using the whole cell patch clamp recording technique with cultured dorsal root ganglion (DRG) neurons from 2-4 day old rat pups. Both metal compounds reduced the current irreversibly in a concentration dependent fashion, reaching a new (lower) steady state within 3 to 5 min after application. Inorganic mercury was more effective in reducing the VACCCs with an IC50 of 7.3 mu M, while the IC50 for methylmercury was 2.6 mu M. But the threshold concentrations were below 0.25 mu M for both metal compounds and the calcium channel currents were reduced by more than 90% with concentrations of 5 mu M and 20 mu M, respectively. The Hill coefficient for both dose-response relationships was calculated as similar to 1. Calcium channel currents were reduced over the entire voltage range, but the current-voltage relation shifted to more positive potentials in a concentration dependent manner, the effect being more pronounced with HgCl2 than with CH3HgCl (1 mu M HgCl2; 10 mV shift, 5 mu M CH3HgCl: 5 mV shift). At higher concentrations (greater than or equal to 2 mu M for HgCl2 and greater than or equal to 10mu M for CH3HgCl) an unidentified membrane current was observed. The inorganic mercury caused an inward current, while the organic mercury compound generated a biphasic current with a transient inward and a long lasting outward component. Our results suggest that mercury compounds affect the electrical properties of neurons and thereby decrease cognitive and motor performance. (C) 1996 Intox Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:19:11