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Titolo:
SPECIFIC METAL-OLIGONUCLEOTIDE BINDING STUDIED BY HIGH-RESOLUTION TANDEM MASS-SPECTROMETRY
Autore:
WU QY; CHENG XH; HOFSTADLER SA; SMITH RD;
Indirizzi:
BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM MOLEC SCI LAB,POB 999 RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM MOLEC SCI LAB RICHLAND WA 99352
Titolo Testata:
Journal of mass spectrometry.
fascicolo: 6, volume: 31, anno: 1996,
pagine: 669 - 675
SICI:
1076-5174(1996)31:6<669:SMBSBH>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA; DISSOCIATION; PLATINUM; CLEAVAGE; SALTS;
Keywords:
COLLISION-INDUCED DISSOCIATION; OLIGONUCLEOTIDES; METAL BINDING; FOURIER TRANSFORM ION CYCLOTRON RESONANCE; ELECTROSPRAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
Q.Y. Wu et al., "SPECIFIC METAL-OLIGONUCLEOTIDE BINDING STUDIED BY HIGH-RESOLUTION TANDEM MASS-SPECTROMETRY", Journal of mass spectrometry., 31(6), 1996, pp. 669-675

Abstract

Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) was used to study the binding of metal ions to two oligonucleotides, d(pGCTTGCATp) and d(TTGGCCCTCCTT). Collision-induced dissociation (CID) of the metal-oligonucleotide complex revealed that metal ions preferentially bound to the central thymine region of the sequence. The most probable binding sites were the phosphodiester backbone since the sum of the maximum number of charge additionfrom the metal ions and the charge state of the whole complex was found to be equal to the number of ionizable protons on the DNA backbone. Although site-specific and sequence-specific binding was observed forall three of the metal ions studied, the binding specificity of UO22ions was significantly greater than for Mg2+ and Na+. These experiments demonstrate that ESI-MS/MS can be applied to study the binding of metal ions and their complexes to oligonucleotides, providing not only information on the number of metal ions binding to the oligonucleotide, but also information related to the binding site(s) and binding specificity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:06:23