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Titolo:
ENDOTHELIAL-CELL SEEDING IMPROVES PATENCY OF SYNTHETIC VASCULAR GRAFTS - MANUAL VERSUS AUTOMATIZED METHOD
Autore:
PASIC M; MULLERGLAUSER W; VONSEGESSER L; ODERMATT B; LACHAT M; TURINA M;
Indirizzi:
DEUTSCH HERZZENTRUM BERLIN,KLIN HERZ THORAX & GEFASSCHIRURG,AUGUSTENBURGER PL 1 D-13353 BERLIN GERMANY UNIV ZURICH HOSP,DEPT SURG,CARDIOVASC SURG CLIN CH-8091 ZURICH SWITZERLAND
Titolo Testata:
European journal of cardio-thoracic surgery
fascicolo: 5, volume: 10, anno: 1996,
pagine: 372 - 379
SICI:
1010-7940(1996)10:5<372:ESIPOS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYTETRAFLUOROETHYLENE GRAFTS;
Keywords:
ENDOTHELIAL CELL SEEDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M. Pasic et al., "ENDOTHELIAL-CELL SEEDING IMPROVES PATENCY OF SYNTHETIC VASCULAR GRAFTS - MANUAL VERSUS AUTOMATIZED METHOD", European journal of cardio-thoracic surgery, 10(5), 1996, pp. 372-379

Abstract

Lack of an endothelial surface is the most important variable causingthe relatively poor patency of synthetic bypass grafts. This study was designed to investigate the effect of endothelial cell seeding on small-diameter Dacron grafts seeded with microvascular endothelial cellsfrom omentum, and to evaluate two methods (manual vs automatized) forone-stage seeding in a canine carotid artery model. In 30 mongrel dogs microvascular endothelial cells were harvested from omentum, either by a manual or an automatized method, and seeded onto 6-mm internal diameter Dacron prostheses prior to the graft interposition into the common carotid arteries. Non-seeded Dacron grafts were used as control grafts. All dogs received dipyridamole (75 mg/day) and acetylsalicylic acid (325 mg/day) for 4 weeks. The prostheses were explanted between 2 and 26 weeks after insertion. The results were assessed by patency, angiography, light and scanning electron microscopy, transmission electron microscopy, and morphometry. Endothelial cell seeding improved the patency rate Significantly, regardless of the seeding methods used, The overall actuarial patency rates at 5, 12, and 26 weeks were 98%, 94%and 94%, respectively, for the seeded Dacron grafts, and 92%, 62% and54%, respectively, for the nonseeded grafts. The automatized method yielded more endothelial cells per gram of omental tissue than the manual method (P = 0.0002), but there was no difference (P = 0.34) betweenthe seeding densities per square centimeter of the graft surface. Theharvesting and seeding by the automatized method took 55 min for the whole procedure, 20 min less than the manual method. We concluded thatone-stage endothelial cell seeding with omental microvascular endothelial cells improved the patency of small-diameter Dacron grafts in a canine model. The automatized method obtained excellent results comparable to the manual procedure, and also reduced the time necessary for the cell seeding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:10:12