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Titolo:
HUMAN COLORECTAL CARCINOMAS SPECIFICALLY ACCUMULATE M(R)42,000 UBIQUITIN-CONJUGATED CYTOKERATIN-8 FRAGMENTS
Autore:
NISHIBORI H; MATSUNO Y; IWAYA K; OSADA T; KUBOMURA N; IWAMATSU A; KOHNO H; SATO S; KITAJIMA M; HIROHASHI S;
Indirizzi:
NATL CANC CTR,RES INST,DIV PATHOL,CHUO KU,5-1-1 TSUKIJI TOKYO 104 JAPAN NATL CANC CTR,RES INST,DIV PATHOL,CHUO KU TOKYO 104 JAPAN KEIO UNIV,SCH MED,DEPT SURG,SHINJUKU KU TOKYO 160 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN KYOWA MEDEX CO LTD,FUJI RES LABS NAGAIZUMI SHIZUOKA 411 JAPAN
Titolo Testata:
Cancer research
fascicolo: 12, volume: 56, anno: 1996,
pagine: 2752 - 2757
SICI:
0008-5472(1996)56:12<2752:HCCSAM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SULFATE-POLYACRYLAMIDE GELS; ATP-DEPENDENT PROTEOLYSIS; INSITU PROTEASE DIGESTION; AMINO-ACID SEQUENCE; INTERMEDIATE FILAMENTS; MALLORY BODIES; CELL-CYCLE; PROTEINS; DEGRADATION; ELECTROPHORESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
H. Nishibori et al., "HUMAN COLORECTAL CARCINOMAS SPECIFICALLY ACCUMULATE M(R)42,000 UBIQUITIN-CONJUGATED CYTOKERATIN-8 FRAGMENTS", Cancer research, 56(12), 1996, pp. 2752-2757

Abstract

Recent studies have shown that various tumor cells accumulate ubiquitin (Ub)-conjugated proteins, the profiles of which differ from those of normal cells. Tn identify the UB-conjugated proteins accumulated specifically by human carcinoma sells, a two-dimensional immunoblot analysis of 31 surgically resected human primary colorectal carcinoma tissues ti as performed using an anti-Ub monoclonal antibody. KM691. Two distinct M(r) 42,000 and 45,000 proteins in tile Triton X-insoluble fractions of carcinoma tissues reacted with this antibody, whereas only one M(r) 45.000 protein reacted in normal tissues. The M(r) 42,000 Ub-conjugated proteins were specific to carcinoma tissues from 25 patients (80.6%). One of the purified 42,000 proteins was digested with Achromobacter protease I. This protein wa identified as a cytokeratin 8 (CK 8) fragment based oil both molecular mass determination and molecular mass searching of Achromobacter protease I-digested fragments of proteins registered in a a protein sequence data base, Two-dimensional immunoblot analysis with an anti-CK 8 antibody confirmed that all cf tire M(r) 42,000 proteins were CK 8 degradation products. These results demonstrate that human colo- rectal carcinomas specifically accumulate M(r) 42,000 Ub-conjugated CK 8 fragments. This accumulation was observed frequently not only in advanced (18/22, 81.8%), but also in early stage casts (7/9, 77.8%), suggesting that it occurs even in the early stages of colorectal carcinoma progression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:34:05