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Titolo:
NOVEL ACRYLAMIDO MONOMERS WITH HIGHER HYDROPHILICITY AND IMPROVED HYDROLYTIC STABILITY .3. DNA SEPARATIONS BY CAPILLARY ELECTROPHORESIS IN POLY(N-ACRYLOYLAMINOPROPANOL)
Autore:
GELFI C; SIMOALFONSO E; SEBASTIANO R; CITTERIO A; RIGHETTI PG;
Indirizzi:
LITA,VIA FRATELLI CERVI 93 I-20090 SEGRATE MILANO ITALY CNR,ITBA I-20133 MILAN ITALY UNIV VALENCIA,FAC QUIM,DEPT QUIM ANALIT E-46100 BURJASSOT SPAIN POLYTECH MILANO,DEPT CHEM MILAN ITALY UNIV CALABRIA,DEPT CELL BIOL RENDE COSENZA ITALY
Titolo Testata:
Electrophoresis
fascicolo: 4, volume: 17, anno: 1996,
pagine: 738 - 743
SICI:
0173-0835(1996)17:4<738:NAMWHH>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARRAY ELECTROPHORESIS; HIGH-SPEED;
Keywords:
N-SUBSTITUTED ACRYLAMIDES; N-ACRYLOYLAMINOPROPANOL; CAPILLARY ELECTROPHORESIS; DNA SEPARATIONS; LIQUID POLYMER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
C. Gelfi et al., "NOVEL ACRYLAMIDO MONOMERS WITH HIGHER HYDROPHILICITY AND IMPROVED HYDROLYTIC STABILITY .3. DNA SEPARATIONS BY CAPILLARY ELECTROPHORESIS IN POLY(N-ACRYLOYLAMINOPROPANOL)", Electrophoresis, 17(4), 1996, pp. 738-743

Abstract

Separation of DNA fragments in a novel polymer network, consisting ofN-acryloylaminopropanol (AAP) is reported. The performance of this novel monomer, as a sieving liquid polymer in capillary zone electrophoresis, was evaluated. In 50 mu m ID capillaries, an 8% solution of poly(AAP) can afford apex-resolution of the 123/124 bp adjacent pair of DNA fragments in marker V, typically unresolved in any poly(acrylamide) formulation. It is proposed that the distal -OH group in the AAP molecule can form transient H-bonds with the DNA double helix. Molecular modeling shows a meandering structure for poly(AAP), lacing the walls ofhalf a cylinder, with kinks protruding at regular intervals, potentially able to fit into the major groove of DNA. Contrary to previously held beliefs, there seems to be a minimum length of the polymer for proper sieving of DNAs. For poly(acrylamides), a weight average molecularmass M(w) 30 000 polymer offers no resolution, whereas a polymer of 250 000 to 400 000 Da exhibits optimum resolving power. For poly(AAP), the optimal length is in excess of 450 000 Da in M(w). Thus, it is shown that both the chemical composition of the monomer and the length ofthe polymer play a subtle, cooperative role in DNA separation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:15:46