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Titolo:
REACTION-MECHANISM OF A NEW GLYCOSYLTREHALOSE-HYDROLYZING ENZYME ISOLATED FROM THE HYPERTHERMOPHILIC ARCHAEUM, SULFOLOBUS-SOLFATARICUS KM1
Autore:
KATO M; MIURA Y; KETTOKU M; KOMEDA T; IWAMATSU A; KOBAYASHI K;
Indirizzi:
KIRIN BREWERY CO LTD,APPL BIORES CTR,3 MIYAHARACHO TAKASAKISHI GUNMA 37012 JAPAN KIRIN BREWERY CO LTD,APPL BIORES CTR TAKASAKISHI GUNMA 37012 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 5, volume: 60, anno: 1996,
pagine: 925 - 928
SICI:
0916-8451(1996)60:5<925:ROANGE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSITE AFFINITIES; POLYMERIZATION;
Keywords:
TREHALOSE; SULFOLOBUS SOLFATARICUS; GLYCOSYLTREHALOSE; ALPHA-AMYLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
M. Kato et al., "REACTION-MECHANISM OF A NEW GLYCOSYLTREHALOSE-HYDROLYZING ENZYME ISOLATED FROM THE HYPERTHERMOPHILIC ARCHAEUM, SULFOLOBUS-SOLFATARICUS KM1", Bioscience, biotechnology, and biochemistry, 60(5), 1996, pp. 925-928

Abstract

Amylolytic activity, which converts soluble starch to alpha,alpha-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic acidophilic archaeum, Sulfolobus solfataricus KM1. Two enzymes, a glycosyltransferase and an alpha-amylase, which were essential forthis activity were identified, The alpha-amylase was purified to homogeneity on SDS-PAGE, The alpha-amylase catalyzed the hydrolysis of glycosyltrehaloses to trehalose, Analysis of the reaction products, kinetic parameters, and experimental findings using H-3-labeled substrates indicated that the alpha-amylase hydrolyzed only the alpha-1,4 glucosidic linkage adjacent to the trehalose unit of the glycosyltrehaloses, Six strains of the Sulfolobaceae family examined were observed to havethe glycosyltrehalose-hydrolyzing enzyme, the alpha-amylase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:57:53