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Titolo:
REACTION-MECHANISM OF A NEW GLYCOSYLTREHALOSE-PRODUCING ENZYME ISOLATED FROM THE HYPERTHERMOPHILIC ARCHAEUM, SULFOLOBUS-SOLFATARICUS KM1
Autore:
KATO M; MIURA Y; KETTOKU M; SHINDO K; IWAMATSU A; KOBAYASHI K;
Indirizzi:
KIRIN BREWERY CO LTD,CTR APPL BIORES,3 MIYAHARACHO TAKASAKISHI GUNMA 37012 JAPAN KIRIN BREWERY CO LTD,CTR APPL BIORES TAKASAKISHI GUNMA 37012 JAPAN KIRIN BREWERY CO LTD,PHARMACEUT RES LABS TAKASAKISHI GUNMA 37012 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 5, volume: 60, anno: 1996,
pagine: 921 - 924
SICI:
0916-8451(1996)60:5<921:ROANGE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSITE AFFINITIES; POLYMERIZATION;
Keywords:
TREHALOSE; SULFOLOBUS SOLFATARICUS; GLYCOSYLTREHALOSE; GLYCOSYLTRANSFERASE; ACIDIANUS BRIERLEYI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
M. Kato et al., "REACTION-MECHANISM OF A NEW GLYCOSYLTREHALOSE-PRODUCING ENZYME ISOLATED FROM THE HYPERTHERMOPHILIC ARCHAEUM, SULFOLOBUS-SOLFATARICUS KM1", Bioscience, biotechnology, and biochemistry, 60(5), 1996, pp. 921-924

Abstract

An amylolytic activity, which converts soluble starch to alpha,alpha-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic, acidophilic archaeum Sulfolobus solfataricus KM1. Two enzymes, a glycosyltransferase and an amylase, which are essential for this activity, were purified to homogeneity. A glycosyltransferase catalyzed the conversion of maltooligosaccharides to glycosyltrehaloses. Based on a detailed analysis of the reaction products, kinetic parameters, and an experiment using H-3-labeled substrates, it was verified thatglycosyltransferase transferred an oligomer segment of maltooligosaccharide to the C1-OH position of glucose, located at the reducing end of the maltooligosaccharide, to produce a glycosyltrehalose having an alpha-1,1 linkage, The reaction appears to be intramolecular, Nine strains of the Sulfolobaceae family were found to have glycosyltransferases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:50:38