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Titolo:
AN ALGORITHM TO ANALYZE THE HYDROLYSIS PATHWAY OF PEPTIDES AND PROTEINS BY SEQUENCE ANALYSES OF UNFRACTIONATED DIGESTION MIXTURES
Autore:
CAPORALE C; SEPE C; CARUSO C; GARZILLO AMV; BUONOCORE V;
Indirizzi:
UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,VIA S CAMILLO LELLIS I-01100 VITERBO ITALY
Titolo Testata:
Computer applications in the biosciences
fascicolo: 2, volume: 12, anno: 1996,
pagine: 81 - 88
SICI:
0266-7061(1996)12:2<81:AATATH>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL-ROLE; IV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
CompuMath Citation Index
CompuMath Citation Index
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
C. Caporale et al., "AN ALGORITHM TO ANALYZE THE HYDROLYSIS PATHWAY OF PEPTIDES AND PROTEINS BY SEQUENCE ANALYSES OF UNFRACTIONATED DIGESTION MIXTURES", Computer applications in the biosciences, 12(2), 1996, pp. 81-88

Abstract

We have designed and implemented on a personal computer a program foridentifying and quantifying the fragments present in a peptide mixture obtained by hydrolysing a polypeptide of known sequence using digesting agents. The qualitative data utilized by the main algorithm consist of the target sequence of the intact molecule and the amino acid residues identified at each step of the automatic sequence analysis of the unfractionated digestion mixture. In this way, the sequence of each fragment present in the mixture is quickly reconstructed Furthermore, if the quantitative data of the amino acid residues identified at eachstep of the sequence analysis are utilized, the program will correlate the sequence of each fragment to its amount. We furnish an example of the application intended for the vapid identification and characterization of the extracellular proteinases produced by a basidiomycete fungus, utilizing the bovine insulin beta-chain as target substrate. A variety of uses for the method ave discussed.

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Documento generato il 20/09/20 alle ore 05:25:29