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Titolo:
A GUINEA-PIG MODEL OF HYPERSENSITIVITY TO ALLERGENIC FRACTIONS OF NATURAL-RUBBER LATEX
Autore:
AAMIR R; SAFADI GS; MANDELIK J; CORNISH K; MELTON AL; PIEN LC; WAGNER WO; BATTISTO JR;
Indirizzi:
CLEVELAND CLIN FDN,RES INST,DEPT IMMUNOL,FFB CLEVELAND OH 44195 CLEVELAND CLIN FDN,RES INST,DEPT IMMUNOL,FFB CLEVELAND OH 44195 CLEVELAND CLIN FDN,SECT ALLERGY & IMMUNOL CLEVELAND OH 44195 CLEVELAND STATE UNIV CLEVELAND OH 44115 USDA ALBANY CA 00000
Titolo Testata:
International archives of allergy and immunology
fascicolo: 2, volume: 110, anno: 1996,
pagine: 187 - 194
SICI:
1018-2438(1996)110:2<187:AGMOHT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELONGATION-FACTOR; HEVEA-BRASILIENSIS; SPINA-BIFIDA; PROTEINS; ANAPHYLAXIS; IGE; IDENTIFICATION; ANTIBODIES; CHILDREN; CONTACT;
Keywords:
NATURAL RUBBER LATEX; LATEX ALLERGY; PASSIVE CUTANEOUS ANAPHYLAXIS; PASSIVE SYSTEMIC ANAPHYLAXIS; IGE; IGG1; GUINEA PIG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
R. Aamir et al., "A GUINEA-PIG MODEL OF HYPERSENSITIVITY TO ALLERGENIC FRACTIONS OF NATURAL-RUBBER LATEX", International archives of allergy and immunology, 110(2), 1996, pp. 187-194

Abstract

Allergy to natural rubber latex is a growing medical problem with life-threatening aspects. The aim of this study was to learn if guinea pigs could serve as a suitable model for immediate-type hypersensitivityto latex. Guinea pigs were immunized either with whole non-ammoniatedlatex extract, or with one of nine SDS-PAGE-separated components. Other animals were injected with electroeluted latex components localizedon gel at 14, 24 and a cluster around 45 kD. Before and after immunization, sera from the animals were examined by ELISA, immunoblots, passive cutaneous anaphylaxis (PCA) and passive systemic anaphylaxis. Latex-specific antibodies were detected by ELISA and immunoblots in sera from all immunized animals. PCA assays showed that the guinea pigs had homocytotropic antibodies dilutible to 1:250. PCA was abolished when sera from animals immunized with allergens in alum were heated at 56 degrees C for 30 min indicating the antibodies were of the E isotype. Passive systemic anaphylaxis was induced in 4 of 10 guinea pigs. The results show that guinea pigs are capable of making antibodies to latex protein components that mediate dermal and systemic anaphylaxis, paralleling the spectrum of clinical and laboratory findings of humans with immediate-type clinical latex hypersensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 02:44:36