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Titolo:
ESTABLISHMENT AND CHARACTERIZATION OF A NEW CANINE B-CELL LEUKEMIA-CELL LINE
Autore:
NAKAICHI M; TAURA Y; KANKI M; MAMBA K; MOMOI Y; TSUJIMOTO H; NAKAMA S;
Indirizzi:
YAMAGUCHI UNIV,DEPT VET SURG YAMAGUCHI 753 JAPAN YAMAGUCHI UNIV,DEPT VET ANAT YAMAGUCHI 753 JAPAN UNIV TOKYO,DEPT VET INTERNAL MED,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Journal of veterinary medical science
fascicolo: 5, volume: 58, anno: 1996,
pagine: 469 - 471
SICI:
0916-7250(1996)58:5<469:EACOAN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY; CULTURE; DOGS;
Keywords:
CANINE; CELL LINE; LYMPHOMA/LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
M. Nakaichi et al., "ESTABLISHMENT AND CHARACTERIZATION OF A NEW CANINE B-CELL LEUKEMIA-CELL LINE", Journal of veterinary medical science, 58(5), 1996, pp. 469-471

Abstract

A new cell line derived from a spontaneous canine leukemia was established and designated GL-1. The cells have been cultured in a floating fashion and passaged for over two years. They were round with rich cytoplasm containing many rough endoplasmic reticula and mitochondria. Peroxidase staining was negative. The nuclei of many cells were round, but segmented nuclei were seen frequently. The doubling time of the cells was 27.3 hr and they had 78 chromosomes. Surface marker analysis using monoclonal antibodies (MABs) and flowcytometry revealed that GL-1 possessed CD45 and surface IgG. However, the cells did not react with MABs detecting T-cell markers. These results indicate that GL-1 has a lymphocytic lineage and is derived from a B-cell leukemia.

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Documento generato il 27/11/20 alle ore 13:56:38