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Titolo:
PLASMA-FREE FATTY-ACIDS DECREASE INSULIN-STIMULATED SKELETAL-MUSCLE GLUCOSE-UPTAKE BY SUPPRESSING GLYCOLYSIS IN CONSCIOUS RATS
Autore:
KIM JK; WI JK; YOUN JH;
Indirizzi:
UNIV SO CALIF,SCH MED,DEPT PHYSIOL & BIOPHYS,1333 SAN PABLO AVE, MMR 626 LOS ANGELES CA 90033 UNIV SO CALIF,SCH MED,DEPT PHYSIOL & BIOPHYS LOS ANGELES CA 90033
Titolo Testata:
Diabetes
fascicolo: 4, volume: 45, anno: 1996,
pagine: 446 - 453
SICI:
0012-1797(1996)45:4<446:PFDISG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT DIABETES-MELLITUS; PYRUVATE-DEHYDROGENASE ACTIVITY; NORMAL HUMANS; METABOLISM; AVAILABILITY; RESISTANCE; GLYCOGEN; INVIVO; CYCLE; STARVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J.K. Kim et al., "PLASMA-FREE FATTY-ACIDS DECREASE INSULIN-STIMULATED SKELETAL-MUSCLE GLUCOSE-UPTAKE BY SUPPRESSING GLYCOLYSIS IN CONSCIOUS RATS", Diabetes, 45(4), 1996, pp. 446-453

Abstract

The effects of elevated plasma free fatty acid (FFA) levels on insulin-stimulated whole-body and skeletal muscle glucose transport, glucoseuptake, glycolysis, and glycogen synthesis were studied in conscious rats during hyperinsulinemic-euglycemic clamps with (n = 26) or without (n = 23) Intralipid and heparin infusion. Whole-body and skeletal muscle glucose uptake, glycolysis, and glycogen synthesis were estimatedusing D-[3-H-3]glucose and 2-[C-14]deoxyglucose (study 1), and glucose transport activity was assessed by analyzing plasma kinetics of L-[C-14]glucose and 3-O-[H-3]-methylglucose (study 2). Plasma FFA levels decreased during the clamps without intralipid but increased above basal during the clamps with Intralipid infusion (P < 0.01 for both). Elevated plasma FFA levels decreased insulin-stimulated whole-body glucoseuptake by similar to 15% and similar to 20% during physiological and maximal insulin clamps, respectively (P < 0.01). Similarly, insulin-stimulated glucose uptake was also decreased in individual skeletal muscles with Intralipid infusion (P < 0.05). The most profound effect of elevated plasma FFA levels was a 30-50% suppression of insulin-stimulated glycolysis in whole body and individual skeletal muscles in both clamps. In contrast, physiological insulin-stimulated glycogen synthesiswas increased with elevated plasma. FFA levels in whole body and individual skeletal muscles (P < 0.05). Glucose-6-phosphate (G-6-P) levelswere increased in soleus and extensors digitorum longus (EDL) muscleswith Intralipid infusion in both clamps (P < 0.05). Intralipid infusion did not alter the time profiles of plasma L-glucose and 3-O-methylglucose after an intravenous injection during maximal insulin clamps, and compartmental analysis indicated no significant effect of elevated FFA levels on glucose transport activity in insulin-sensitive tissues (P > 0.05). Thus, elevated plasma FFA decreased insulin-stimulated glucose uptake in skeletal muscle by suppressing glycolysis and increasing G-6-P levels. These findings suggest that the classic glucose-fatty acid cycle was the predominant mechanism underlying the inhibitory effect of FFA on skeletal muscle glucose uptake.

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Documento generato il 03/04/20 alle ore 12:51:37