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Titolo:
BIOCHEMICAL-CHARACTERIZATION AND PROTEIN-KINASE-C DEPENDENCY OF MONOKINE-INDUCING ACTIVITIES OF TOXOPLASMA-GONDII
Autore:
GRUNVALD E; CHIARAMONTE M; HIENY S; WYSOCKA M; TRINCHIERI G; VOGEL SN; GAZZINELLI RT; SHER A;
Indirizzi:
NIAID,IMMUNOBIOL SECT,PARASIT DIS LAB,NIH,BLDG 4,ROOM 126 BETHESDA MD20892 NIAID,IMMUNOBIOL SECT,PARASIT DIS LAB,NIH BETHESDA MD 20892 WISTAR INST ANAT & BIOL PHILADELPHIA PA 19104 UNIFORMED SERV UNIV HLTH SCI,DEPT MICROBIOL & IMMUNOL BETHESDA MD 20814 UNIV FED MINAS GERAIS,DEPT BIOCHEM & IMMUNOL BR-30161970 BELO HORIZONT MG BRAZIL
Titolo Testata:
Infection and immunity
fascicolo: 6, volume: 64, anno: 1996,
pagine: 2010 - 2018
SICI:
0019-9567(1996)64:6<2010:BAPDOM>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MESSENGER-RNA EXPRESSION; CELL STIMULATORY FACTOR; NATURAL-KILLER-CELLS; INTERFERON-GAMMA; IFN-GAMMA; MACROPHAGE ACTIVATION; PLASMODIUM-CHABAUDI; MURINE MACROPHAGES; ACCESSORY CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
E. Grunvald et al., "BIOCHEMICAL-CHARACTERIZATION AND PROTEIN-KINASE-C DEPENDENCY OF MONOKINE-INDUCING ACTIVITIES OF TOXOPLASMA-GONDII", Infection and immunity, 64(6), 1996, pp. 2010-2018

Abstract

Previous reports have indicated that the early induction of interleukin-12 (IL-12), tumor necrosis factor alpha (TNF-alpha), IL-1 beta, andIL-10 is crucial for the establishment and regulation of host cell-mediated immunity to the intracellular protozoan parasite Toxoplasma gondii. In this study, we demonstrate that a soluble tachyzoite extract (soluble tachyzoite antigen) can trigger the expression of these four monokines by murine inflammatory macrophages. Further characterization revealed that the parasite molecules in soluble tachyzoite antigen responsible for monokine induction are heat stable at 100 degrees C but differ in sensitivity to protease digestion. Thus, the tachyzoite factors that stimulate TNF-alpha and IL-1 beta expression were found to be more resistant to treatment with proteinase K than those responsible for IL-12 and IL-10 induction. Similarly, while the factors responsiblefor the induction of all four monokines were found to be sensitive toperiodate oxidation, the TNF-alpha-stimulating activity was partiallyresistant to treatment with the compound at a low concentration (1 mM). A further dichotomy in monokine induction signals was inferred fromexperiments with isoquinoline sulfonamide protein kinase inhibitors. The latter work suggested that the pathways for TNF-alpha and IL-1 beta are protein kinase C dependent, while expression of IL-12 and expression of IL-10 share distinct signal transduction mechanisms involving other kinases. Together, these data argue that monokine induction by T, gondii is mediated by glycoproteins that may belong to distinct groups in terms of their biochemical properties and intracellular signaling pathways.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 23:54:25