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Titolo:
HUMAN ERYTHROCYTES BEARING ELECTROINSERTED CD4 NEUTRALIZE INFECTION IN-VITRO BY PRIMARY ISOLATES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-I
Autore:
TOSI PF; SCHWARTZ D; SHARMA U; MOUNEIMNE Y; HANNIG J; LI GR; MCKINLEY G; GRIECO M; FLEXNER CW; LAZARTE J; NORSE D; NICOLAU C; VOLSKY DJ;
Indirizzi:
ST LUKES ROOSEVELT HOSP,MOLEC VIROL LAB,ANTENUCCI RES BLDG,ROOM 709,432 W 58TH ST NEW YORK NY 10019 ST LUKES ROOSEVELT HOSP,MOLEC VIROL LAB NEW YORK NY 10019 CTR BLOOD RES LAB BOSTON MA 00000 HARVARD UNIV,SCH MED BOSTON MA 00000 JOHNS HOPKINS UNIV,SCH HYG & PUBL HLTH,DEPT MOLEC MICROBIOL & IMMUNOLBALTIMORE MD 00000 ST LUKES ROOSEVELT HOSP,HIV AIDS CLIN TRIALS PROGRAM,DIV ALLERGY & INFECT DIS NEW YORK NY 10019 COLUMBIA UNIV,COLL PHYS & SURG NEW YORK NY 00000
Titolo Testata:
Blood
fascicolo: 11, volume: 87, anno: 1996,
pagine: 4839 - 4844
SICI:
0006-4971(1996)87:11<4839:HEBECN>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SOLUBLE CD4; AIDS-RELATED COMPLEX; HIV-INFECTION; CELLS; MEMBRANE; LYMPHOCYTES; REPLICATION; EXPRESSION; TROPISM; THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
P.F. Tosi et al., "HUMAN ERYTHROCYTES BEARING ELECTROINSERTED CD4 NEUTRALIZE INFECTION IN-VITRO BY PRIMARY ISOLATES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-I", Blood, 87(11), 1996, pp. 4839-4844

Abstract

Human erythrocytes bearing electroinserted full-length CD4 (RBC-CD4) can bind and fuse with a laboratory strain of human immunodeficiency virus type 1 (HIV-1) or with T cells infected by HIV-1. Here we show that RBC-CD4 neutralize primary HIV-1 strains in an assay of cocultivation of peripheral blood mononuclear cells (PBMC) from HIV-1-infected persons with uninfected PBMC. RBC-CD4 inhibited viral p24 core antigen accumulation in these cocultures up to 10,000-fold compared with RBC alone. Viral p24 accumulation was inhibited equally well when measured in culture supernatants or in cell extracts. The inhibition was dose-dependent and long-lived. Two types of recombinant CD4 tested in parallel were largely ineffective. The neutralization of primary HIV-1 by RBC-CD4 in vitro was demonstrated in PBMC cultures from 21 of a total of 23 patients tested at two independent sites. RBC-CD4 may offer a routeto blocking HIV-1 infection in vivo. (C) 1996 by The American Societyof Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:50:56