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Titolo:
EX-VIVO EXPANSION WITH STEM-CELL FACTOR AND INTERLEUKIN-11 AUGMENTS BOTH SHORT-TERM RECOVERY POSTTRANSPLANT AND THE ABILITY TO SERIALLY TRANSPLANT MARROW
Autore:
HOLYOAKE TL; FRESHNEY MG; MCNAIR L; PARKER AN; MCKAY PJ; STEWARD WP; FITZSIMONS E; GRAHAM GJ; PRAGNELL IB;
Indirizzi:
CRC,BEATSON LABS,BEATSON INST CANC RES,GARSCUBE ESTATE,SWITCHBACK RD GLASGOW G61 1BD LANARK SCOTLAND ROYAL ALEXANDRIA HOSP,DEPT HAEMATOL PAISLEY RENFREW SCOTLAND GLASGOW WESTERN INFIRM,BEATSON ONCOL CTR GLASGOW LANARK SCOTLAND MONKLANDS DIST HOSP,DEPT HAEMATOL LANARK SCOTLAND
Titolo Testata:
Blood
fascicolo: 11, volume: 87, anno: 1996,
pagine: 4589 - 4595
SICI:
0006-4971(1996)87:11<4589:EEWSFA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; HEMATOPOIETIC RECONSTITUTION; MURINE HEMATOPOIESIS; GROWTH-FACTORS; KIT-LIGAND; C-KIT; CULTURE; MICE; INVITRO; ENHANCEMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
T.L. Holyoake et al., "EX-VIVO EXPANSION WITH STEM-CELL FACTOR AND INTERLEUKIN-11 AUGMENTS BOTH SHORT-TERM RECOVERY POSTTRANSPLANT AND THE ABILITY TO SERIALLY TRANSPLANT MARROW", Blood, 87(11), 1996, pp. 4589-4595

Abstract

The characterization of many cytokines involved in the control of hematopoiesis has led to intense investigation into their potential use in ex vivo culture to expand progenitor numbers. We have established the optimum ex vivo culture conditions that allow substantial amplification of transient engrafting murine stem cells and which, simultaneously, augment the ability to sustain serial bone marrow transplantation (BMT). Short-term incubation of unfractionated BM cells in liquid culture with stem cell factor (SCF) and interleukin-ll (IL-ll) produced a 50-fold amplification of clonogenic multipotential progenitors (CFU-A). Following such ex vivo expansion, substantially fewer cells were required doses above threshold for engraftment, BM cells expanded ex vivo resulted in significantly more rapid hematopoietic recovery. In a serial transplantation model, unmanipulated BM was only able to consistently sustain secondary BMT recipients, but BM expanded ex vivo has sustained quaternary BMT recipients that remain alive and well more than 140 days after 4 degrees BMT. These results show augmentation of both short-term recovery posttransplant and the ability to serially transplant marrow by preincubation in culture with SCF and IL-ll. (C) 1996 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 08:34:29