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Titolo:
USING MULTIPLEX PCR AMPLIFICATION AND TYPING KITS FOR THE ANALYSIS OFDNA EVIDENCE IN A SERIAL KILLER CASE
Autore:
HOCHMEISTER MN; BUDOWLE B; EISENBERG A; BORER UV; DIRNHOFER R;
Indirizzi:
UNIV BERN,INST LEGAL MED,DNA IDENT LAB,BUEHLSTR 20 CH-3012 BERN SWITZERLAND FED BUR INVEST ACAD,LAB DIV,FORENS SCI RES & TRAINING CTR QUANTICO VA00000 UNIV N TEXAS,HLTH SCI CTR,DEPT PATHOL,DNA IDENT LAB FT WORTH TX 00000
Titolo Testata:
Journal of forensic sciences
fascicolo: 1, volume: 41, anno: 1996,
pagine: 155 - 162
SICI:
0022-1198(1996)41:1<155:UMPAAT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; TETRANUCLEOTIDE REPEAT POLYMORPHISM; SKELETAL REMAINS; SINGLE HAIRS; IDENTIFICATION; SEQUENCES; LOCUS; BONE; WAR;
Keywords:
FORENSIC SCIENCE; CRIMINALISTICS; HUMAN IDENTIFICATION; DEOXYRIBONUCLEIC ACID (DNA); POLYMERASE CHAIN REACTION (PCR); GENETIC TYPING; HLA DQA1; LDLR; GYPA; HBGG; D7S8; GC; SHORT TANDEM REPEAT (STR) LOCI; TH01; TPOX; CSF1PO; AMELOGENIN; SEXING; REVERSE PATERNITY; HAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
M.N. Hochmeister et al., "USING MULTIPLEX PCR AMPLIFICATION AND TYPING KITS FOR THE ANALYSIS OFDNA EVIDENCE IN A SERIAL KILLER CASE", Journal of forensic sciences, 41(1), 1996, pp. 155-162

Abstract

Analysis of DNA evidence in a serial killer case was performed using the AmpliType(R) HLA-DQ alpha-, AmpliType(R) PM-, and the GenePrint(TM) STR Multiplex System PCR Amplification Kits. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. DNA profiles from a single hair with attached sheath material, recovered from underneath the seat cover of the suspect's car seat were compared with DNA profiles derived from reference head hairs from a homicide victim. From the evidentiary sample only 9 ng of human DNA could berecovered. In a sample, where the quantity of DNA becomes a critical issue a powerful route is the simultaneous amplification of several loci (multiplex PCR). This is the first report where commercially available multiplex PCR amplification and typing kits have been introduced for the analysis of DNA evidence in a serial killer case and the analysis has been admitted in court.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 07:35:05