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Titolo:
CELL-DENSITY-DEPENDENT EXPRESSION OF THE BETA-ADRENERGIC RESPONSE BY EPIDERMAL GROWTH-FACTOR (EGF) IN PRIMARY CULTURES OF ADULT-RAT HEPATOCYTES
Autore:
OGIHARA M;
Indirizzi:
JOSAI UNIV,FAC PHARMACEUT SCI,BIOCHEM PHARMACOL GRP,1-1 KEYAKIDAI SAKADO SAITAMA 35002 JAPAN
Titolo Testata:
Biological & pharmaceutical bulletin
fascicolo: 5, volume: 19, anno: 1996,
pagine: 752 - 757
SICI:
0918-6158(1996)19:5<752:CEOTBR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE ADENYLATE-CYCLASE; ISLET-ACTIVATING PROTEIN; PERTUSSIS TOXIN; DNA-SYNTHESIS; CYCLIC-AMP; RECEPTORS; LIVER; PHOSPHORYLASE; MODULATION; GLUCAGON;
Keywords:
BETA-ADRENERGIC RESPONSE; EGF; PRIMARY HEPATOCYTE CULTURE; METAPROTERENOL; UK-14304;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Ogihara, "CELL-DENSITY-DEPENDENT EXPRESSION OF THE BETA-ADRENERGIC RESPONSE BY EPIDERMAL GROWTH-FACTOR (EGF) IN PRIMARY CULTURES OF ADULT-RAT HEPATOCYTES", Biological & pharmaceutical bulletin, 19(5), 1996, pp. 752-757

Abstract

The effects of epidermal growth factor (EGF) and cell density on the appearance of beta-adrenergic responses were examined in primary cultures of adult rat hepatocytes, The beta-adrenergic response was measured as the ability to accumulate cAMP by beta(2)-agonist metaproterenol in monolayers that had been cultured without or with 20 ng/ml EGF, Hepatocytes cultured with EGF at a high cell density (1.0 x 10(5) cells/cm(2)) showed a relatively lower response to 10 mu M metaproterenol. Incontrast, when cultured at a low cell density (3.3 x 10(4) cells/cm(2)) with EGF, the cells showed a higher response to the beta-adrenergicagonist. These responses were blocked by the beta-adrenergic antagonist propranolol (10 mu M). The beta-adrenergic response increased rapidly with culture time, The addition of cycloheximide (5 mu M) to the culture abolished the expression of beta-adrenergic response, The enhanced beta-adrenergic response by 20 ng/ml EGF was partially inhibited bythe addition of cytochalasin B (20 mu M) to the culture. The cAMP-producing response to metaproterenol (10 mu M) was dose-dependently inhibited by the specific alpha(2)-agonist UK-14304, Pretreatment of the hepatocytes with pertussis toxin (100 ng/ml) potentiated the beta-adrenergic response, These results demonstrate that augmented beta-adrenergic responsiveness can be acquired by adult rat hepatocytes cultured with 20 ng/ml EGF at a low cell density, and the beta-adrenergic responseinvolves de novo synthesis of protein(s). The results also show that significant alpha(2)- and beta-adrenergic responses coexist in the primary cultures of adult rat hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:21:25