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Titolo:
AMPLIFICATION OF THE 11Q13 REGION IN HUMAN CARCINOMA CELL-LINES - A MECHANISTIC VIEW
Autore:
ROELOFS H; SCHUURING E; WIEGANT J; MICHALIDES R; GIPHARTGASSLER M;
Indirizzi:
SYLVIUS LABS,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS SYLVIUS LABS,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS GORLAEUS LABS,DEPT MOLEC GENET LEIDEN NETHERLANDS LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS 2300 RA LEIDEN NETHERLANDS ANTONI VAN LEEUWENHOEK HOSP,NETHERLANDS CANC INST,DIV TUMOR BIOL AMSTERDAM NETHERLANDS
Titolo Testata:
Genes, chromosomes & cancer
fascicolo: 2, volume: 7, anno: 1993,
pagine: 74 - 84
SICI:
1045-2257(1993)7:2<74:AOT1RI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-MYC ONCOGENE; SUBMICROSCOPIC EXTRACHROMOSOMAL ELEMENTS; DOUBLE MINUTE CHROMOSOMES; GENE AMPLIFICATION; INVERTED DUPLICATION; SQUAMOUS-CELL; HAMSTER-CELLS; CIRCULAR DNA; CAD GENE; LOCUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
H. Roelofs et al., "AMPLIFICATION OF THE 11Q13 REGION IN HUMAN CARCINOMA CELL-LINES - A MECHANISTIC VIEW", Genes, chromosomes & cancer, 7(2), 1993, pp. 74-84

Abstract

We previously proposed that a local duplication, not the loss of the subsequently amplified marker from its original site, might be the first step in gene amplification in human cells. It is important to investigate this issue in naturally occurring amplification and when copy numbers are relatively low. We have examined the location of single-copy and amplified 11q13 sequences in cell lines from human breast cancers and squamous cell carcinomas using fluorescence in situ hybridization both with a probe specific for the 11q13 amplifying region and with a chromosome 11-specific library. We show that in most cell lines the 11q13 amplicons are physically linked to chromosome II or to a chromosome derived from chromosome 11 by various rearrangements near the 11q13 region. In none of the cell lines were interstitial deletions of 11q13 detected. These results indicate that 11q13 amplification in human tumor cells generally does not involve deletion as the initial step. One cell line with chromosomally located amplified 11q13 sequences contained double minutes that harbored the MYC gene but no 11q13 sequences. This suggests that the genetic outcome and the mechanism of gene amplification are probably dependent on specific DNA sequences rather than on the origin of the cells.

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Documento generato il 28/11/20 alle ore 15:46:28