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Titolo:
ALTERING CELL FATES IN SEA-URCHIN EMBRYOS BY OVEREXPRESSING SPOTX, ANORTHODENTICLE-RELATED PROTEIN
Autore:
MAO CA; WIKRAMANAYAKE AH; GAN L; CHUANG CK; SUMMERS RG; KLEIN WH;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANC CTR,DEPT BIOCHEM & MOLEC BIOL HOUSTON TX 77030 STATE UNIVERSITY NEW YORK BUFFALO,SCH MED,DEPT ANAT SCI BUFFALO NY 14214
Titolo Testata:
Development
fascicolo: 5, volume: 122, anno: 1996,
pagine: 1489 - 1498
SICI:
0950-1991(1996)122:5<1489:ACFISE>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DROSOPHILA HEAD DEVELOPMENT; GENE-EXPRESSION; STRONGYLOCENTROTUS-PURPURATUS; SPATIAL PATTERNS; SPEC GENES; ACTIN GENE; ECTODERM; DIFFERENTIATION; HOMEODOMAIN; ELEMENTS;
Keywords:
SEA URCHIN; EMBRYO DEVELOPMENT; HOMEOBOX PROTEIN; ORTHODENTICLE/OTX-GENES; SPOTX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Mao et al., "ALTERING CELL FATES IN SEA-URCHIN EMBRYOS BY OVEREXPRESSING SPOTX, ANORTHODENTICLE-RELATED PROTEIN", Development, 122(5), 1996, pp. 1489-1498

Abstract

While many general features of cell fate specification in the sea urchin embryo are understood, specific factors associated with these events remain unidentified, SpOtx, an orthodenticle-related protein, has been implicated as a transcriptional activator of the aboral ectoderm-specific Spec2a gene. Here, we present evidence that SpOtx has the potential to alter cell fates, SpOtx was found in the cytoplasm of early cleavage stage embryos and was translocated into nuclei between the 60-and 120-cell stage, coincident with Spec gene activation. Eggs injected with SpOtx mRNA developed into epithelial halls of aboral ectoderm suggesting that SpOtx redirected nonaboral ectoderm cells to an aboralectoderm fate, At least three distinct domains on SpOtx, the homeoboxand regions in the N-terminal and C-terminal halves of the protein, were required for the morphological alterations, These same N-terminal and C-terminal regions were shown to be transactivation domains in a yeast transactivation assay, indicating that the biological effects of overexpressing SpOtx were due to its action as a transcription factor,Our results suggest that SpOtx is involved in aboral ectoderm differentiation by activating aboral ectoderm-specific genes and that modulating its expression can lead to changes in cell fate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 11:07:48