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Titolo: REGULATION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN RAT ANTERIOR-PITUITARY
Autore: SAKAI K; HORIBA N; SAKAI Y; TOZAWA F; DEMURA H; SUDA T;
- Indirizzi:
- HIROSAKI UNIV,SCH MED,DIV 3,DEPT MED,5 ZAIFU CHO HIROSAKI AOMORI 036 JAPAN TOKYO WOMENS MED COLL,INST CLIN ENDOCRINOL,DEPT MED TOKYO 162 JAPAN
- Titolo Testata:
- Endocrinology
fascicolo: 5,
volume: 137,
anno: 1996,
pagine: 1758 - 1763
- SICI:
- 0013-7227(1996)137:5<1758:ROCFM>2.0.ZU;2-G
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- CHEMICAL CROSS-LINKING; FACTOR CRF RECEPTORS; ARGININE VASOPRESSIN; MEDIAN-EMINENCE; ADRENALECTOMY; BINDING; BRAIN; EXPRESSION; HORMONE; GLAND;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 32
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- K. Sakai et al., "REGULATION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN RAT ANTERIOR-PITUITARY", Endocrinology, 137(5), 1996, pp. 1758-1763
Abstract
Adrenalectomy (ADX) leads to a decrease in the number of CRF-binding sites in the rat anterior pituitary (AP). However, the molecular mechanisms of CRF receptor (CRF-R) regulation are unknown. In the present study, we analyzed the effects of ADX on pituitary CRF-R1 messenger RNA(mRNA) levels in vivo and the direct effects of CRF, arginine vasopressin (AVP), and glucocorticoid, the levels of which are altered by ADX, on CRF-R1 mRNA levels in vitro. The mRNA level was determined by Northern blot analysis using a rat brain CRF-R1 complementary RNA probe. The CRF-R1 level in AP fell to 20% of the sham level 1 day after ADX and returned to the sham level after 14 days. In cultured rat AP cells,treatment with CRF, AVP, and dexamethasone led to significant reductions in CRF-R1 mRNA, with maximal inhibition to 32%, 22%, and 37% of control levels, respectively. The time course of CRF-R1 mRNA reduction varied depending on the drug, with effects detectable as early as 1 h after treatment. These findings indicate that elevated portal CRF and AVP levels may contribute to the decrease in CRF-R1 mRNA soon after ADX. A decrease in mRNA levels, in turn, may lead to a decrease in CRF-R1protein on corticotrophs.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 01:00:18