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Titolo:
THE STR GENE-CLUSTER FOR THE BIOSYNTHESIS OF 5'-HYDROXYSTREPTOMYCIN IN STREPTOMYCES-GLAUCESCENS GLA.0 (ETH-22794) - NEW OPERONS AND EVIDENCE FOR PATHWAY SPECIFIC REGULATION BY STRR
Autore:
BEYER S; DISTLER J; PIEPERSBERG W;
Indirizzi:
BERG UNIV GESAMTHSCH WUPPERTAL,FB9 MIKROBIOL,GAUSS STR 20 D-42097 WUPPERTAL GERMANY BERG UNIV GESAMTHSCH WUPPERTAL,FB9 MIKROBIOL D-42097 WUPPERTAL GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 6, volume: 250, anno: 1996,
pagine: 775 - 784
SICI:
0026-8925(1996)250:6<775:TSGFTB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; MULTIDRUG-RESISTANCE; ESCHERICHIA-COLI; CYSTIC-FIBROSIS; CLONING; GRISEUS; BLUENSOMYCIN; TRANSPORT; PROTEINS; PROMOTER;
Keywords:
STREPTOMYCIN BIOSYNTHESIS; STR GENE REGULATION; ACTIVATOR STRR; STREPTOMYCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
S. Beyer et al., "THE STR GENE-CLUSTER FOR THE BIOSYNTHESIS OF 5'-HYDROXYSTREPTOMYCIN IN STREPTOMYCES-GLAUCESCENS GLA.0 (ETH-22794) - NEW OPERONS AND EVIDENCE FOR PATHWAY SPECIFIC REGULATION BY STRR", MGG. Molecular & general genetics, 250(6), 1996, pp. 775-784

Abstract

Two divergently oriented operons, strXU and strVW, located within thegene cluster for 5'-hydroxystreptomycin (5'-OH-Sm) biosynthesis in Streptomyces glaucescens strain GLA.0 (ETH 22794), were analysed by DNA sequencing and transcription/regulation studies. Three genes, strU andstrVW, are conserved in a similar arrangement but in a different location within the str/sts gene cluster of the Sm-producing strain S. griseus N2-3-11. The four putative products resemble NDP-4-ketohexose 3,5-epimerases (StrX, M(r) 20.2 kDa), NAD(P)-dependent oxidoreductases (StrU, 45.6 kDa), and ABC-transporters (StrV, 61.8 kDa; StrW, 63.4 kDa). These genes are apparently involved in the biosynthesis of 5'-OH-Sm because the promoters of both operons are activated in trans by the activator StrR of S. griseus N2-3-11, when cloned in S. lividans 66 TK23. A sequence motif resembling the consensus sequence GTTCGActC(N)(11)CagTcGAAc for binding of StrR was identified within the intergenic region of strX and strV. Specific binding of StrR to this site was demonstrated by gel retardation assays using purified HisTag-StrR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 01:04:50