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Titolo:
CELL-DENSITY-DEPENDENT EXPRESSION OF THE ALPHA-2-ADRENERGIC RESPONSE BY EPIDERMAL GROWTH-FACTOR (EGF) IN PRIMARY CULTURES OF ADULT-RAT HEPATOCYTES
Autore:
OGIHARA M;
Indirizzi:
JOSAI UNIV,FAC PHARMACEUT SCI,BIOCHEM PHARMACOL GRP,1-1 KEYAKIDAI SAKADO SAITAMA 35002 JAPAN
Titolo Testata:
Biological & pharmaceutical bulletin
fascicolo: 4, volume: 19, anno: 1996,
pagine: 518 - 524
SICI:
0918-6158(1996)19:4<518:CEOTAR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE REGULATORY PROTEIN; ALPHA-ADRENERGIC RECEPTORS; ADENYLATE-CYCLASE ACTIVITY; CYCLIC-AMP; ACTIVATION; FORSKOLIN; SUBTYPES; BINDING; AGONIST;
Keywords:
ALPHA(2)-ADRENERGIC RESPONSE; EPIDERMAL GROWTH FACTOR; HEPATOCYTE PRIMARY CULTURE; UK-14304; OXYMETAZOLINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Ogihara, "CELL-DENSITY-DEPENDENT EXPRESSION OF THE ALPHA-2-ADRENERGIC RESPONSE BY EPIDERMAL GROWTH-FACTOR (EGF) IN PRIMARY CULTURES OF ADULT-RAT HEPATOCYTES", Biological & pharmaceutical bulletin, 19(4), 1996, pp. 518-524

Abstract

Effects of epidermal growth factor (EGF) on the expression of alpha(2)-adrenergic responses were examined in primary cultures of adult rat hepatocytes, The alpha(2)-responses were assessed by the inhibition ofthe rate of forskolin-stimulated cAMP formation by the selective alpha(2)-adrenergic agonists, oxymetazoline and UK-14304, Hepatocytes cultured with EGF (20 ng/ml) at a high cell density (1.0 x 10(5)/cm(2)) showed almost no response to the alpha(2)-adrenergic agonists, oxymetazoline and UK-14304 (1-100 mu M). In contrast, when cultured at a low cell density (3.3 x 10(4) cells/cm(2)) with EGF, forskolin-stimulated cAMP production was inhibited by oxymetazoline and UK-14304 in a dose-dependent manner, The alpha(2)-response was blocked by the alpha(2)-antagonist yohimbine (10 mu M). It was also reversed by treatment of hepatocytes with pertussis toxin (100 ng/ml). In addition, the effects of EGF on the appearance of alpha(2)-responses were almost completely inhibited by treatment of the hepatocytes with genistein (10 mu M) or cytochalasin B (10 mu M). The alpha(2)-response was abolished when cycloheximide (5 mu M) was added to the cultures. These results demonstrate that when cultured at a lolv cell density with EGF, adult rat hepatocytes acquire a significant alpha(2)-adrenergic response. The expression of this alpha(2)-response is associated with de novo protein synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 13:56:48