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Titolo:
EFFECTS OF RETINOIC ACID ON CELL-DIFFERENTIATION AND REVERSION TOWARDNORMAL IN HUMAN ENDOMETRIAL ADENOCARCINOMA (RL95-2) CELLS
Autore:
CARTER CA; POGRIBNY M; DAVIDSON A; JACKSON CD; MCGARRITY LJ; MORRIS SM;
Indirizzi:
UNIV ARKANSAS MED SCI HOSP,DEPT OBSTET & GYNECOL,SLOT 518,4301 W MARKHAM LITTLE ROCK AR 72205 UNIV ARKANSAS MED SCI HOSP,DEPT PEDIAT LITTLE ROCK AR 72205 NATL CTR TOXICOL RES,DIV NUTR TOXICOL JEFFERSON AR 72079 NATL CTR TOXICOL RES,DIV GENET TOXICOL JEFFERSON AR 72079
Titolo Testata:
Anticancer research
fascicolo: 1, volume: 16, anno: 1996,
pagine: 17 - 24
SICI:
0250-7005(1996)16:1<17:EORAOC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER PATIENTS; INHIBITION; CARCINOMA; EXPRESSION; TAMOXIFEN; ORGANIZATION; EPITHELIUM; INDUCTION; INVASION; CULTURE;
Keywords:
RETINOIC ACID; RL95-2 CELLS; F-ACTIN; ENDOMETRIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Carter et al., "EFFECTS OF RETINOIC ACID ON CELL-DIFFERENTIATION AND REVERSION TOWARDNORMAL IN HUMAN ENDOMETRIAL ADENOCARCINOMA (RL95-2) CELLS", Anticancer research, 16(1), 1996, pp. 17-24

Abstract

Background: All-trans retinoic acid is currently used in clinical trials in combination with tamoxifen to treat breast cancer, and 13-cis retinoic acid is used with a-interferon to treat metastatic endometrialcancer. We examined the effects of all-trans retinoic acid 13-cis RA alone on endometrial adenocarcinoma (RL95-2) cells to investigate the cell biological mechanisms by which retinoic acid may reduce the metastatic phenotype and induce differentiation. Methods: RL95-2 cells wereseeded onto 4-chamber plastic slides and treated with 13-cis retinoicacid or all-trans retinoic acid at 0.5 mu M, 1 mu M and 5 mu M doses for 90 minutes at 37 degrees C and stained for F-actin. Results: Untreated RL95-2 cells exhibited staining of disrupted aggregates of F-actin only near the cell periphery. Cells treated with the three doses of 13-cis retinoic acid exhibited a dramatic reorganization of F-actin throughout the cells. When cells were treated with 0.5 mu M all-trans retinoic acid, actin filaments reorganized. Cells treated with 1 mu M all-trans retinoic acid and 5 mu M all-trans retinoic acid displayed increased organization of F-actin and cell size increased. The percentageof S-phase cells increased at the high doses of retinoic acid treatment. This effect was apparently transient, since retinoic acid did not significantly affect cell growth. Conclusion: An organized cytoskeleton and an increase in cell size are associated with differentiation. Wesuggest that retinoic acid exerts its effects on these transformed cells by reorganizing actin filaments, and inducing differentiation, thus inducing a more stationary phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 10:23:34