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Titolo:
DNA ADDUCT FORMATION IN RELATION TO LYMPHOCYTE MUTATIONS AND LUNG-TUMOR INDUCTION IN F344 RATS TREATED WITH THE ENVIRONMENTAL-POLLUTANT 1,6-DINITROPYRENE
Autore:
SMITH BA; FULLERTON NF; AIDOO A; HEFLICH RH; BELAND FA;
Indirizzi:
NATL CTR TOXICOL RES,DIV BIOCHEM TOXICOL,HFT-110 JEFFERSON AR 72079 NATL CTR TOXICOL RES,DIV BIOCHEM TOXICOL,HFT-110 JEFFERSON AR 72079 NATL CTR TOXICOL RES,DIV GENET TOXICOL JEFFERSON AR 72079 UNIV ARKANSAS MED SCI HOSP,DEPT BIOCHEM & MOLEC BIOL LITTLE ROCK AR 72205
Titolo Testata:
Environmental health perspectives
, volume: 99, anno: 1993,
pagine: 277 - 280
SICI:
0091-6765(1993)99:<277:DAFIRT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARCINOGENICITY; BENZO)A>PYRENE; CONDENSATE; EXHAUST; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
B.A. Smith et al., "DNA ADDUCT FORMATION IN RELATION TO LYMPHOCYTE MUTATIONS AND LUNG-TUMOR INDUCTION IN F344 RATS TREATED WITH THE ENVIRONMENTAL-POLLUTANT 1,6-DINITROPYRENE", Environmental health perspectives, 99, 1993, pp. 277-280

Abstract

Epidemiological studies suggest an association between exposure to diesel emissions and an increased incidence of lung and bladder cancer in humans. Of the compounds associated with diesel emissions, 1,6-dinitropyrene is a particularly potent mutagen and carcinogen. In these experiments we administered [4,5,9,10-H-3]1,6-dinitropyrene (30 or 100 mug) directly to the lungs of F344 rats according to a protocol known toinduce lung tumors and characterized the DNA adducts present in the target tissue. In addition, we examined the adducts present in spleen lymphocytes and assayed for the induction of mutations at the hypoxanthine-guanine phosphoribosyltransferase locus in these cells as measuredby the frequency of 6-thioguanine-resistant (TG(r)) T-lymphocytes. Adduct formation was detected in both lung and spleen lymphocyte DNA, with the extent of binding being dose-dependent in the lymphocytes but not the lung. P-32-Postlabeling analyses indicated the formation of a major DNA adduct, N-(deoxyguanosin-8-yl)-1-amino-6-nitropyrene, in bothtissues. 1,6-Dinitropyrene treatment resulted in a dose-dependent increase in TG(r) T-lymphocytes, with the increase being detected for at least 21 weeks after treatment. These data indicate that 1,6-dinitropyrene is metabolically activated by nitroreduction to form DNA adducts in both the target tissue and spleen lymphocytes and that a tumorigenic dose results in a significant induction of TG(r) T-lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:46:18