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Titolo:
EFFECTS OF P-2-PURINOCEPTOR ANTAGONISTS ON ECTO-NUCLEOTIDASE ACTIVITYOF GUINEA-PIG VAS-DEFERENS CULTURED SMOOTH-MUSCLE CELLS
Autore:
ZIGANSHIN AU; ZIGANSHINA LE; BODIN P; BAILEY D; BURNSTOCK G;
Indirizzi:
UNIV LONDON UNIV COLL,DEPT ANAT & DEV BIOL,GOWER ST LONDON WC1E 6BT ENGLAND UNIV LONDON UNIV COLL,CTR NEUROSCI LONDON WC1E 6BT ENGLAND
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 4, volume: 36, anno: 1995,
pagine: 863 - 869
SICI:
1039-9712(1995)36:4<863:EOPAOE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
URINARY-BLADDER; ATPASE; MEMBRANE; IDENTIFICATION; SYNAPTOSOMES; INHIBITORS; RESPONSES;
Keywords:
P-2-PURINOCEPTOR ANTAGONISTS; ECTO-NUCLEOTIDASE; GUINEA-PIG VAS DEFERENS; SMOOTH MUSCLE CULTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
A.U. Ziganshin et al., "EFFECTS OF P-2-PURINOCEPTOR ANTAGONISTS ON ECTO-NUCLEOTIDASE ACTIVITYOF GUINEA-PIG VAS-DEFERENS CULTURED SMOOTH-MUSCLE CELLS", Biochemistry and molecular biology international, 36(4), 1995, pp. 863-869

Abstract

Ecto-nucleotidase activity was studied on primary cultures of guinea-pig vas deferens smooth muscle cells by measuring the inorganic phosphate (P-i) production using ATP as a substrate. The ecto-nucleotidase was insensitive to ouabain, oligomycin, sodium azide, p-nitrophenyl phosphate and beta-glycerophosphate. Enzyme activity was highly dependenton either Ca2+ or Mg2+. Antagonists of P-2X-purinoceptors, pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid, 4,4'-diisothiocyanatostilbene-2,2'-disulphon suramin and pyridoxal-5-phosphate, significantly inhibited ecto-nucleotidase activity. In contrast, the P-1-purinoceptorantagonists, 8-p-sulphophenyl theophylline and 1,3-dipropyl-8-cyclopentyl-xanthine, did not affect the enzyme activity. Thus, when P-2-purinoceptors are studied by testing agonists and antagonists potencies, the inhibition of ecto-nucleotidase activity by currently available P-2-purinoceptor antagonists should be taken into account.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:00:18