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Titolo:
DIRECT OBSERVATION AND QUANTIFICATION OF MACROPHAGE CHEMOATTRACTION TO THE GROWTH-FACTOR CSF-1
Autore:
WEBB SE; POLLARD JW; JONES GE;
Indirizzi:
UNIV LONDON KINGS COLL,RANDALL INST,26-29 DRURY LANE LONDON WC2B 5RL ENGLAND UNIV LONDON KINGS COLL,RANDALL INST LONDON WC2B 5RL ENGLAND YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT DEV & MOL BIOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT OBSTET & GYNECOL BRONX NY 10461
Titolo Testata:
Journal of Cell Science
, volume: 109, anno: 1996,
parte:, 4
pagine: 793 - 803
SICI:
0021-9533(1996)109:<793:DOAQOM>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; FACTOR-I; POLYMORPHONUCLEAR LEUKOCYTES; CHEMOTACTIC FACTORS; HUMAN-NEUTROPHILS; CELL POLARITY; FACTOR-ALPHA; RECEPTOR; PROLIFERATION;
Keywords:
MACROPHAGE; CYTOKINE; GROWTH FACTOR; CSF-1; M-CSF; TNF; CELL MOTILITY; CHEMOTAXIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
S.E. Webb et al., "DIRECT OBSERVATION AND QUANTIFICATION OF MACROPHAGE CHEMOATTRACTION TO THE GROWTH-FACTOR CSF-1", Journal of Cell Science, 109, 1996, pp. 793-803

Abstract

The cloned mouse macrophage cell line, BAC1.25F, resembles primary macrophages in its dependence on colony stimulating factor-1 (CSF-1) forboth viability and proliferation. Re-addition of CSF-1 to cytokine-deprived cells, which are rounded with diffusely organised F-actin, stimulates rapid cell spreading and cell polarisation. Using the Dunn chemotaxis chamber the movement of stimulated macrophages was monitored over a 2 hour period. Cells restimulated with 1.32 nM human recombinant CSF-1 migrated at a mean rate of 7.71 mu m per hour, but showed no directional preferences. In a linear concentration gradient of CSF-1, cytokine-deprived cells were again stimulated to migrate and the mean rate of cell motility, at 6.88 mu m per hour, was not significantly different from that measured in an isotropic environment of CSF-1. However,there was a strong preference for the cells to orientate so that their long axes aligned with the CSF-1 gradient and they migrated preferentially towards the source of CSF-1. Migrating cells contained abundantF-actin within the leading lamellae as judged by confocal imaging of fluorescent phalloidin, but the actin was not arranged into stress fibre-like structures. These data support the proposition that CSF-1 is both a chemokinetic and chemotactic agent for macrophages. Tumour necrosis factor (TNF-alpha) failed to stimulate cell migration and thus wasneither chemokinetic nor a chemotactic agent. However, cells exposed to a dual concentration gradient of both TNF-alpha and CSF-1 did migrate successfully, although the chemotactic response to CSF-1 was abolished.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:53:08