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Titolo:
GOLD-CONJUGATED REAGENTS FOR THE LABELING OF CARBOHYDRATE-RECOGNITIONDOMAINS AND GLYCOCONJUGATES ON NEMATODE SURFACES
Autore:
SHARON E; SPIEGEL Y;
Indirizzi:
AGR RES ORG,VOLCANI CTR,DEPT NEMATOL IL-50250 BET DAGAN ISRAEL AGR RES ORG,VOLCANI CTR,DEPT NEMATOL IL-50250 BET DAGAN ISRAEL
Titolo Testata:
Journal of nematology
fascicolo: 1, volume: 28, anno: 1996,
pagine: 124 - 127
SICI:
0022-300X(1996)28:1<124:GRFTLO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT PARASITIC NEMATODES; LECTIN BINDING-SITES; BODY WALL; MELOIDOGYNE; RESIDUES;
Keywords:
CONCANAVALIN A; FLUORESCENT; GLYCOCONJUGATE; HETERODERA AVENAE; HETERODERA LATIPONS; LECTIN; MELOIDOGYNE JAVANICA; NEMATODE; NEOGLYCOPROTEIN; STEINERNEMA; CARPOCAPSAE; STEINERNEMA GLASERI; WHEAT GERM AGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
E. Sharon e Y. Spiegel, "GOLD-CONJUGATED REAGENTS FOR THE LABELING OF CARBOHYDRATE-RECOGNITIONDOMAINS AND GLYCOCONJUGATES ON NEMATODE SURFACES", Journal of nematology, 28(1), 1996, pp. 124-127

Abstract

Various fluorescent conjugated lectins have been used For the detection of glycoconjugates on nematode surfaces under light microscopy. Several problems have been experienced with these reagents including penetration of the cuticle by fluorescent lectins, non-glycoconjugate specificity, strong nematode autofluorescence at the emission wavelength of the fluorescent dye, and prevention of persistent visualization due to rapid quenching of the fluorescent components. Gold-conjugated reagents combined with silver enhancement alleviated these difficulties when working with three phytonematode species (Heterodera avenae, H. latipons, and Meloidogyne javanica) and two entomopathogenic species (Steinernema carpocapsae and S. glaseri) under light-microscopy visualization of binding by fluorescent lectins and neoglycoproteins. Moreover, gold-conjugated reagents resulted in stable bindings that enabled long-term observations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 03:32:01