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Titolo:
NONCYTOPATHOGENIC AND CYTOPATHOGENIC BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUSES DO NOT AFFECT IN-VITRO EMBRYONIC-DEVELOPMENT INTO THE BLASTOCYST STAGE
Autore:
TSUBOI T; IMADA T;
Indirizzi:
NATL INST ANIM HLTH,TOHOKU BRANCH LAB,31 UMINAI KAMIKITA AOMORI 03925JAPAN
Titolo Testata:
Veterinary microbiology
fascicolo: 1-2, volume: 49, anno: 1996,
pagine: 127 - 134
SICI:
0378-1135(1996)49:1-2<127:NACBVD>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
BVD VIRUS; CATTLE; INFECTION; PATHOGENESIS; INVITRO; BORN; CALF;
Keywords:
BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUS; CATTLE, VIRUSES; BLASTOCYST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
T. Tsuboi e T. Imada, "NONCYTOPATHOGENIC AND CYTOPATHOGENIC BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUSES DO NOT AFFECT IN-VITRO EMBRYONIC-DEVELOPMENT INTO THE BLASTOCYST STAGE", Veterinary microbiology, 49(1-2), 1996, pp. 127-134

Abstract

Bovine oocytes enclosed within follicular epithelial (FE) cells were exposed to noncytopathogenic or cytopathogenic strains of bovine viraldiarrhea-mucosal disease virus (BVD-MDV). After culture at 39 degreesC in humidified air with 5% CO2 for 24 h, the oocytes matured and then in vitro fertilization was performed. Some of the fertilized oocytesdeveloped into blastocysts from day 8 to day 10 (day 1: date of insemination), and the rate of development to blastocysts was the same as for the unexposed control oocytes. In the developmental medium, each strain of BVD-MDV was present at 10(3) to 10(6) TCID50/0.25 ml from day 3 to day 10, respectively and the virus was isolated from the FE cellsat high titers and from the embryos at low titers on day 10. BVD-MDV antigen was detected in the cytoplasm of the FE cells by indirect immunofluorescence. These findings indicate that BVD-MDV replicates well in FE cells. The results of this study suggest that BVD-MDV replicationin cells around embryos has no effect on bovine embryo development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:55:17