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Titolo:
UTILITY OF A RAPID POLYMERASE CHAIN-REACTION PANEL FOR THE DETECTION OF MOLECULAR-CHANGES IN B-CELL LYMPHOMA
Autore:
ABDELREHEIM FA; EDWARDS E; ARBER DA;
Indirizzi:
CITY HOPE NATL MED CTR,DIV PATHOL,1500 E DUARTE RD DUARTE CA 91010 CITY HOPE NATL MED CTR,DIV PATHOL DUARTE CA 91010 TEXAS A&M UNIV,HLTH SCI CTR,COLL MED,SCOTT & WHITE MEM HOSP & CLIN,DEPT PATHOL TEMPLE TX 76508
Titolo Testata:
Archives of pathology and laboratory medicine
fascicolo: 4, volume: 120, anno: 1996,
pagine: 357 - 363
SICI:
0003-9985(1996)120:4<357:UOARPC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; EMBEDDED SECTIONS; BIOPSY SPECIMENS; BCL-2 GENE; DISEASE; TRANSLOCATION; TISSUE; REARRANGEMENTS; POPULATIONS; DIAGNOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
F.A. Abdelreheim et al., "UTILITY OF A RAPID POLYMERASE CHAIN-REACTION PANEL FOR THE DETECTION OF MOLECULAR-CHANGES IN B-CELL LYMPHOMA", Archives of pathology and laboratory medicine, 120(4), 1996, pp. 357-363

Abstract

Objective.-To evaluate the utility of a polymerase chain reaction (PCR)-based, B-cell-related molecular panel in the diagnostic workup of hematologic specimens. Design, Setting, and Patients.-A PCR panel was applied to 89 specimens from 87 patients, including 55 cases (57 specimens) of known monoclonal B-cell lymphoma, 10 cases of Hodgkin's disease, 2 cases of T-cell lymphoma, and 20 specimens of polyclonal reactivelymphoid tissues. The panel comprised a seminested PCR procedure for immunoglobulin heavy-chain (IgH) gene rearrangement and un-nested PCR detection of t(14;18) and t(11;14). Results.-lmmunoglobulin heavy-chain was detected in 63%, evidence of t(14;18) in 35%, and evidence of t(11;14) in 3.5% of all the B-cell lymphoma cases. Seventy-seven percentof all cases demonstrated IgH- and/or bcl-2-associated translocationsusing these primers. The IgH primers alone detected clonality in 82% (28/34) of the nonfollicular lymphomas and 35% (8/23) of the follicular lymphomas, with no false positives in the non-B-cell lymphoma specimens. The addition of two primer sets for the detection of both IgH andbcl-2/JH significantly improved the detection of molecular abnormalities in the follicular lymphoma group from 35% to 70% (16/23). However,no change was noted in the overall detection rate for the nonfollicular lymphoma group. Adding primers for bcl-1/JH did not change the overall detection rate for either group. Conclusions.-Seminested PCR for IgH detected monoclonality in the majority of the nonfollicular lymphomas and is a valuable diagnostic tool in this group. The combination ofdifferent primer sets for the detection of IgH gene rearrangement andbcl-2/JH is most desirable for follicular lymphomas.

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Documento generato il 10/07/20 alle ore 16:49:14