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Titolo:
ADENOSINE-DEAMINASE (ADA) ISOENZYME ANALYSIS IN PLEURAL EFFUSIONS - DIAGNOSTIC ROLE, AND RELEVANCE TO THE ORIGIN OF INCREASED ADA IN TUBERCULOUS PLEURISY
Autore:
VALDES L; SANJOSE E; ALVAREZ D; VALLE JM;
Indirizzi:
URBANIZAC PARQUE MONTOUTO,C EMILIA PARDO BAZAN 26 E-15894 LA CORUNA SPAIN COMPLEJO HOSP UNIV SANTIAGO,HOSP CONXO,SECC NEUMOL SANTIAGO COMPOSTE SPAIN COMPLEJO HOSP UNIV SANTIAGO,HOSP CONXO,SERV BIOQUIM SANTIAGO COMPOSTESPAIN
Titolo Testata:
The European respiratory journal
fascicolo: 4, volume: 9, anno: 1996,
pagine: 747 - 751
SICI:
0903-1936(1996)9:4<747:A(IAIP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM;
Keywords:
ADENOSINE DEAMINASE; ADENOSINE DEAMINASE ISOENZYMES; PLEURAL EFFUSION; TUBERCULOUS PLEURISY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
L. Valdes et al., "ADENOSINE-DEAMINASE (ADA) ISOENZYME ANALYSIS IN PLEURAL EFFUSIONS - DIAGNOSTIC ROLE, AND RELEVANCE TO THE ORIGIN OF INCREASED ADA IN TUBERCULOUS PLEURISY", The European respiratory journal, 9(4), 1996, pp. 747-751

Abstract

The rise in adenosine deaminase (ADA) activity in the pleural fluid of tuberculous pleurisy patients, though used for diagnosis, is of unknown origin. In this work, we determined ADA activity and the activities of 2'-deoxyadenosine deaminase and ADA-2 in 350 patients. We also considered whether the results throw light on the origin of high pleuralfluid ADA in tuberculous pleurisy and estimated the diagnostic efficiency of 2'-deoxyadenosine deaminase, ADA-2 and total ADA activities with and without the inclusion of the 2'-deoxyadenosine deaminase/ADA activity ratio in a combined criterion. The 350 pleural effusions were classified by previously established criteria as transudates (60 males/18 females) or as tuberculous (49 males/27 females), neoplastic (50 males/39 females), parapneumonic (36 males/19 females), empyematous (11 males/3 females), or miscellaneous (25 males/13 females) exudates. Total ADA, ADA-2 and 2'-deoxyadenosine deaminase activities were, respectively, 127.5+/-2.9, 103+/-29.5 and 42.8+/-14 U . L(-1) in tuberculous exudates. With diagnostic thresholds of 47, 40 and 22 U . L(-1) respectively, the sensitivities of ADA, ADA-2 and 2'-deoxyadenosine deaminase for tuberculosis were 100, 100 and 95%; their specificities 91, 96 and 92%; and their efficiencies 93, 97 and 93%, respectively. One hundred and one effusions (all 76 tuberculous, 12 neoplastic, 4 parapneumonic and 9 empyematous exudates) had total ADA levels >47 U . L(-1); of these, 8 neoplastic, 1 parapneumonic and all the tuberculous exudates had a 2'-deoxyadenosine deaminase/ADA activity ratio <0.49. The criterion of simultaneously having ADA >47 U . L(-1), ADA-2 >40 U . L(-1) and a 2'-deoxyadenosine deaminase/ADA activity ratio <0.49 was satisfiedby all the tuberculous effusions but only eight others (all neoplastic) (sensitivity 100%, specificity 97%, efficiency 98%). We conclude that: 1) high total ADA activity in tuberculous pleural effusions is duemainly to an increase in ADA-2, and, therefore, originated from the only known source monocytes and macrophages; 2) ADA-2 was a more efficient diagnostic marker of tuberculous pleurisy than total ADA activity,although the difference was not statistically significant; and 3) among effusions with high total ADA the 2'-deoxyadenosine deaminase/ADA activity ratio differentiates tuberculous effusions from empyemas and parapneumonic effusions, but fails to discriminate well between tuberculous and neoplastic effusions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 06:19:53