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Titolo:
DIFFERENTIATION OF FOLLICULAR DENDRITIC CELLS AND FULL ANTIBODY-RESPONSES REQUIRE TUMOR-NECROSIS-FACTOR RECEPTOR-1 SIGNALING
Autore:
LEHIR M; BLUETHMANN H; KOSCOVILBOIS MH; MULLER M; DIPADOVA F; MOORE M; RYFFEL B; EUGSTER HP;
Indirizzi:
ETH ZURICH,INST TOXICOL,SWISS INST TECHNOL,SCHORENSTR 16 CH-8603 SCHWERZENBACH SWITZERLAND F HOFFMANN LA ROCHE & CO LTD,PHARMACEUT RES GENE TECHNOL CH-4002 BASEL SWITZERLAND GLAXO INST MOLEC BIOL SA CH-1228 PLANCHES LES OUAT SWITZERLAND GENENTECH INC SAN FRANCISCO CA 94080
Titolo Testata:
The Journal of experimental medicine
fascicolo: 5, volume: 183, anno: 1996,
pagine: 2367 - 2372
SICI:
0022-1007(1996)183:5<2367:DOFDCA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTOGENES; RESISTANT; INFECTION; ANTIGEN; MEMORY; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M. Lehir et al., "DIFFERENTIATION OF FOLLICULAR DENDRITIC CELLS AND FULL ANTIBODY-RESPONSES REQUIRE TUMOR-NECROSIS-FACTOR RECEPTOR-1 SIGNALING", The Journal of experimental medicine, 183(5), 1996, pp. 2367-2372

Abstract

Using mice double deficient for tumor necrosis factor (TNF) and lymphotoxin alpha (LT alpha), we demonstrated that TNF and/or LT alpha are necessary for development of a normal splenic microarchitecture and for isotype switch after immunization with sheep red blood cells (SRBC). In the present study, we extended these observations by determining which TNF receptor (TNFR) is involved in morphological and functional differentiation of the spleen. Spleen morphology and antibody response were investigated in wild-type, TNFR1(-/-), TNFR2(-/-), and TNF/LT alpha(-/-) mice immunized with SRBC. TNF/LT alpha(-/-) mice, which have acomplete disruption of the TNF/LT alpha signaling system including the LT beta-receptor pathway, displayed an abnormal microarchitecture, and isotype switch did not take place. TNFR1(-/-) and TNFR2(-/-) mice displayed a normal spleen microarchitecture and mounted an IgM and IgG antibody response to SRBC. However, the IgG production in TNFR1(-/-) mice Was minimal, with titers leveling off 6 d after immunization. In this strain, immunofluorescence revealed a lack of follicular dendriticcells (FDC) network, detected with FDC-M1 as well as anti-CR1, and a lack of germinal centers, detected with peanut agglutinin. In conclusion, whereas normal splenic microarchitecture and isotype switch might require the LT beta receptor, differentiation of FDC network, development of germinal centers, and full IgG response depend on signaling viaTNFR1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/21 alle ore 02:41:42