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Titolo:
2-AMINOPURINE SELECTIVELY INHIBITS SPLICING OF TUMOR-NECROSIS-FACTOR-ALPHA MESSENGER-RNA
Autore:
JARROUS N; OSMAN F; KAEMPFER R;
Indirizzi:
HEBREW UNIV JERUSALEM,DEPT MOLEC VIROL,SCH MED IL-91120 JERUSALEM ISRAEL HEBREW UNIV JERUSALEM,DEPT MOLEC VIROL,SCH MED IL-91120 JERUSALEM ISRAEL
Titolo Testata:
Molecular and cellular biology
fascicolo: 6, volume: 16, anno: 1996,
pagine: 2814 - 2822
SICI:
0270-7306(1996)16:6<2814:2SISOT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; PROTEIN-KINASE; MESSENGER-RNA; TRANSCRIPTIONAL ACTIVATION; BINDING PROTEIN; FACTOR-BETA; C-FOS; INTERFERON; INITIATION; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
N. Jarrous et al., "2-AMINOPURINE SELECTIVELY INHIBITS SPLICING OF TUMOR-NECROSIS-FACTOR-ALPHA MESSENGER-RNA", Molecular and cellular biology, 16(6), 1996, pp. 2814-2822

Abstract

2-Aminopurine (2-AP) inhibits specific kinases that phosphorylate thea subunit of eukaryotic translation initiation factor 2. One of these, PKR, is also involved in signal transduction. We show here that 2-APselectively inhibits expression of tumor necrosis factor alpha (TNF-alpha) mRNA in primary human lymphoid cells. 2-AP does not inhibit transcription of the human TNF-alpha gene, nor does it affect mRNA stability. Instead, the flow of short-lived precursor transcripts into matureTNF-alpha mRNA is blocked. When 2-AP is present during induction, unspliced TNF-alpha precursor transcripts accumulate at the expense of mRNA. Using RNase protection analysis with genomic probes for different exon-intron junctions, we show that 2-AP blocks splicing of TNF-alpha mRNA. Neither the TNF-beta nor the interleukin-1 beta gene shows such regulation. 2-AP also inhibits splicing of precursor RNA transcribed from an exogenous human TNF-alpha gene. Sequences within this gene thusconfer sensitivity to 2-AP. Yet, control is not exerted at a specificsplice site. Our results reveal the involvement of a 2-AP-sensitive component, expressed in functional form before induction, in the splicing of TNF-alpha mRNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 06:03:52