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Titolo:
DETECTION OF SINGLE-COPY GENES AND CHROMOSOME REARRANGEMENTS IN PETUNIA-HYBRIDA BY FLUORESCENCE IN-SITU HYBRIDIZATION
Autore:
FRANSZ PF; STAM M; MONTIJN B; TENHOOPEN R; WIEGANT J; KOOTER JM; OUD O; NANNINGA N;
Indirizzi:
UNIV AMSTERDAM,INST MOLEC CELL BIOL,BIOCENTRUM AMSTERDAM,KRISLAAN 3161098 SM AMSTERDAM NETHERLANDS UNIV AMSTERDAM,INST MOLEC CELL BIOL,BIOCENTRUM AMSTERDAM 1098 SM AMSTERDAM NETHERLANDS FREE UNIV AMSTERDAM,INST MOLEC BIOL SCI,BIOCENTRUM AMSTERDAM 1081 HV AMSTERDAM NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Plant journal
fascicolo: 5, volume: 9, anno: 1996,
pagine: 767 - 774
SICI:
0960-7412(1996)9:5<767:DOSGAC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSITU HYBRIDIZATION; METAPHASE CHROMOSOMES; HOMOLOGOUS GENES; DNA-SEQUENCES; SYNTHASE; CHALCONE; SUPPRESSION; EXPRESSION; TRANS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
P.F. Fransz et al., "DETECTION OF SINGLE-COPY GENES AND CHROMOSOME REARRANGEMENTS IN PETUNIA-HYBRIDA BY FLUORESCENCE IN-SITU HYBRIDIZATION", Plant journal, 9(5), 1996, pp. 767-774

Abstract

DNA sequences homologous to single-copy genes were labelled with biotinylated dUTP or digoxygenin-labelled dUTP and hybridized to chromosome spreads. The hybridization signals were visualized with fluorescent avidin- or antibody-conjugates. This method allowed the detection of DNA targets on metaphase chromosomes as small as 1.4 kb. The hybridization signals were identified as fluorescent spots on both sister chromatids. Using an 188 rDNA probe as marker to identify chromosomes II andIII it was possible to assign single-copy genes to these chromosomes. In the line V30 the endogenous chalcone synthase gene (chsA) was mapped at the distal end of the short arm of chromosome 5. The cDNA probe for this single-copy gene was 1.4 kb. In contrast, in the lines Mitchell and V26 chsA was localized at the distal end of the long arm of chromosome 3, suggesting that a chromosomal rearrangement had taken place. In a transformed Petunia uidA, transgenes were detected using a 2.7 kb probe. One transgene was mapped on one of the homologues of chromosome II proximal to the ribosomal genes. This homologue could be distinguished from the other by having the ribosomal genes at the distal endof the long arm. Using multicolour fluorescence in site hybridizationit was shown that it is possible to detect the endogenous chsA genes and both transgenes simultaneously.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:15:46