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Titolo:
IFN-GAMMA UP-REGULATES EXPRESSION OF THE COMPLEMENT COMPONENTS C3 ANDC4 BY STABILIZATION OF MESSENGER-RNA
Autore:
MITCHELL TJ; NAUGHTON M; NORSWORTHY P; DAVIES KA; WALPORT MJ; MORLEY BJ;
Indirizzi:
HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,RHEUMATOL UNIT,DUCANE RD LONDON W12 0NN ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,RHEUMATOL UNIT LONDON W12 0NNENGLAND
Titolo Testata:
The Journal of immunology
fascicolo: 11, volume: 156, anno: 1996,
pagine: 4429 - 4434
SICI:
0022-1767(1996)156:11<4429:IUEOTC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; TUBULAR EPITHELIAL-CELLS; INTERFERON-GAMMA; GENE-EXPRESSION; FACTOR-B; MEDIATES STIMULATION; 3RD COMPONENT; BIOSYNTHESIS; PROTEINS; INTERLEUKIN-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
T.J. Mitchell et al., "IFN-GAMMA UP-REGULATES EXPRESSION OF THE COMPLEMENT COMPONENTS C3 ANDC4 BY STABILIZATION OF MESSENGER-RNA", The Journal of immunology, 156(11), 1996, pp. 4429-4434

Abstract

We investigated the mechanisms underlying the regulation of complement genes C3 and C4 by IFN-gamma. IFN-gamma (500 U/ml, 24 h incubation) increased steady state mRNA levels for both C3 and C4 in three different cell types (Hep G2, U937, and primary fibroblasts). The response toIFN-gamma in Hep G2 cells was time and dose dependent. At all doses of IFN-gamma and at all incubation times, the transcription rate for these two genes, determined by nuclear run-on assays, was reduced (0.3 +/- 0.1; unstimulated rate = 1.00). The t(1/2) of mRNA for C3 and C4 inunstimulated cells was 1.8 +/- 0.3 h and 2.2 +/- 0.2 h, respectively. After high-dose IFN-gamma stimulation, both C3 and C4 mRNA levels remained at 100% with respect to baseline at 5 h, but after 12 h, levels fell to 13 +/- 2% (C3) and 8 +/- 3% (C4) of baseline values, giving a half-life for these mRNA species of between 5 and 12 h. IFN-gamma stimulation increased C3 and C4 protein synthesis measured at 24 h. We suggest that it is the increase in mRNA stability that is the major effector mechanism by which IFN-gamma regulates C3 and C4 gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:24:35