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Titolo:
DEMONSTRATION OF HIGH-AFFINITY MN2-CEREVISIAE - SPECIFICITY AND KINETICS( UPTAKE IN SACCHAROMYCES)
Autore:
GADD GM; LAURENCE OS;
Indirizzi:
UNIV DUNDEE,DEPT BIOL SCI DUNDEE DD1 4HN SCOTLAND
Titolo Testata:
Microbiology
, volume: 142, anno: 1996,
parte:, 5
pagine: 1159 - 1167
SICI:
1350-0872(1996)142:<1159:DOHM-S>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-UTILIS; CELLULAR-DISTRIBUTION; CALCIUM HOMEOSTASIS; MEMBRANE-TRANSPORT; COPPER UPTAKE; MANGANESE; YEAST; CARLSBERGENSIS; ACCUMULATION; POTASSIUM;
Keywords:
MN2+; MG2+; SACCHAROMYCES CEREVISIAE; TRANSPORT KINETICS; DIVALENT CATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
G.M. Gadd e O.S. Laurence, "DEMONSTRATION OF HIGH-AFFINITY MN2-CEREVISIAE - SPECIFICITY AND KINETICS( UPTAKE IN SACCHAROMYCES)", Microbiology, 142, 1996, pp. 1159-1167

Abstract

The existence of multiple transport systems for Mn2+ in Saccharomycescerevisiae has been demonstrated in this study. Mn2+ (supplied as MnCl2) was accumulated by S. cerevisiae at all Mn2+ concentrations examined (25 nM-1 mM) but a log-log plot of uptake rates and total amounts accumulated revealed the existence of at least two Mn2+-concentration-dependent transport systems. Over a low Mn2+ concentration range (25-1000 nM), high-affinity Mn2+ uptake occurred with a K-m value of 0.3 mu M, while transformation of kinetic data obtained over the concentration range 5-200 mu M revealed another system with a K-m of 62 mu M. Meaningful kinetic analyses were not possible at higher Mn2+ concentrations because of toxicity: only about 30% of cells remained viable after 30 min incubation with 1000 mu M MnCl2. Release of K+ accompanied Mn2+ accumulation and this increased with increasing Mn2+ concentration. However, even in non-toxic Mn2+ concentrations, the ratio of Mn2+ uptaketo K+ release greatly exceeded electroneutral stoichiometric exchange. In 50 mu M MnCl2, the ratio was 1:123 and this increased to 1:2670 in 1000 mu M MnCl2, a toxic concentration. External Mg2+ was found to decrease Mn2+ accumulation at all concentrations examined, but to differing extents. Over the low Mn2+ concentration range (5-200 mu M), Mg2competitively inhibited Mn2+ uptake with a half-maximal inhibitory concentration, K-i, of 5.5 mu M Mg2+. However, even in the presence of a50-fold excess of Mg2+, inhibition of Mn2+ uptake was of the order of72% and it appears that the cellular requirement for Mn2+ could be maintained even in the presence of such a large excess of Mg2+. Over thehigh Mn2+ concentration range (5-200 mu M), the K-i for Mg2+ was 25.2mu M. At low Mn2+ concentrations, Zn2+ and Co2+, but not Cd2+, inhibited Mn2+ uptake, which indicated that the high-affinity Mn2+ uptake system was of low specificity, while at higher Mn2+ concentrations, where the lower-affinity Mn2+ transport system operated, inhibition was less marked. However, competition studies with potentially toxic metal cations were complicated due to toxic effects, particularly noticeable at 50 mu M Co2+ and Cd2+.

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Documento generato il 17/01/21 alle ore 17:18:34