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Titolo:
CHARACTERIZATION OF RAT LYMPHOCYTE PRIMARY CULTURE FOR THE DEVELOPMENT OF AN IN-VITRO MUTAGENESIS ASSAY - EFFECT OF INTERLEUKIN-2 AND 2-MERCAPTOETHANOL ON THE ACTIVITIES OF INTERMEDIARY METABOLISM ENZYMES AND CELL-PROLIFERATION
Autore:
AIDOO A; FEUERS RJ; LYNCOOK LE; BISHOP ME; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DIV GENET TOXICOL,HFT 120 JEFFERSON AR 72079
Titolo Testata:
Cell biology and toxicology
fascicolo: 2, volume: 12, anno: 1996,
pagine: 79 - 87
SICI:
0742-2091(1996)12:2<79:CORLPC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-BLOOD; PROTEIN; MODULATION; ACTIVATION; INDUCTION; INVIVO; PHYTOHEMAGGLUTININ; CARCINOGENICITY; PHOSPHORYLATION; RESPONSIVENESS;
Keywords:
INTERMEDIARY METABOLISM ENZYMES; MITOGEN STIMULATION; RAT LYMPHOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
A. Aidoo et al., "CHARACTERIZATION OF RAT LYMPHOCYTE PRIMARY CULTURE FOR THE DEVELOPMENT OF AN IN-VITRO MUTAGENESIS ASSAY - EFFECT OF INTERLEUKIN-2 AND 2-MERCAPTOETHANOL ON THE ACTIVITIES OF INTERMEDIARY METABOLISM ENZYMES AND CELL-PROLIFERATION", Cell biology and toxicology, 12(2), 1996, pp. 79-87

Abstract

Efficient energy utilization is essential for cell growth; in an attempt to improve the growth conditions of the rat T-lymphocyte culture model for potential use in studying the mutagenic activity of carcinogens in vitro, we have investigated the effects of phytohemagglutinin (PHA), interleukin-2 (IL-2) and 2-mercaptoethanol (2-ME) on the activities of intermediary metabolism enzymes and cell proliferation. Isolatedlymphocytes were cultured in the presence and absence of PHA, IL-2, or 2-ME. The intermediary metabolism enzymes investigated were glutamate dehydrogenase, glutamate-pyruvate transaminase, malate dehydrogenase, isocitrate dehydrogenase, lactate dehydrogenase, pyruvate kinase, and fatty acid synthetase (FAS). Measurable activity of all enzymes investigated, except for FAS, was detected in PHA-stimulated cells cultured with IL-2 or 2-ME. The unstimulated lymphocytes had significantly lower enzyme activity than stimulated cells. The combination of all three agents showed increased enzyme activity. This increase in activity brought about by the combination of the three agents was not reproducedby either agent acting alone. In general, the increase in enzyme activity correlated with cell proliferation as measured by [H-3]thymidine uptake in PHA-stimulated cultures containing IL-2 and/or 2-ME. The results suggest that the addition of exogenous IL-2 and 2-ME enhances metabolic function and may be beneficial in in vitro culture of rat lymphocytes.

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Documento generato il 03/06/20 alle ore 09:48:55