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Titolo:
PROGLUCAGON PROCESSING IN ISLET AND INTESTINAL-CELL LINES
Autore:
TUCKER JD; DHANVANTARI S; BRUBAKER PL;
Indirizzi:
UNIV TORONTO,DEPT PHYSIOL,ROOM 3366 MED SCI BLDG TORONTO ON M5S 1A8 CANADA UNIV TORONTO,DEPT PHYSIOL TORONTO ON M5S 1A8 CANADA
Titolo Testata:
Regulatory peptides
fascicolo: 1, volume: 62, anno: 1996,
pagine: 29 - 35
SICI:
0167-0115(1996)62:1<29:PPIIAI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GASTRIC-INHIBITORY POLYPEPTIDE; GLUCAGON GENE-TRANSCRIPTION; PEPTIDE-1 7-36 AMIDE; GASTROINTESTINAL-TRACT; ENZYMES PAM; RAT; PANCREAS; EXPRESSION; LOCALIZATION; FORMS;
Keywords:
PROGLUCAGON PROCESSING; GLUCAGON; GLICENTIN; OXYNTOMODULIN; GLUCAGON-LIKE PEPTIDE-1; PROHORMONE CONVERTASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Tucker et al., "PROGLUCAGON PROCESSING IN ISLET AND INTESTINAL-CELL LINES", Regulatory peptides, 62(1), 1996, pp. 29-35

Abstract

To investigate the factors involved in the post-translational processing of proglucagon, we have examined the proglucagon-derived peptides (PGDPs) expressed in normal mouse pancreas and intestine, as well as in both islet (InR1-G9, RIN 1056A) and intestinal (STC-1) cell lines. N-terminal proglucagon processing was similar to that of normal mouse pancreas in InR1-G9 cells, but differed in RIN 1056A and STC-1 cells, which contained significant amounts of glucagon as well as the intestinal PGDPs, glicentin and oxyntomodulin. The C-terminal end of proglucagon was processed to small amounts of glucagon-like peptide-1 in InR1-G9 and RIN 1056A cells, as in normal pancreas, while processing was more extensive in both STC-1 cells and normal intestine. Northern blot analysis of mRNA transcripts for the prohormone convertases, PC1 and PC2, in the 3 cell lines demonstrated correlations between PC2 and the presence of glucagon, as well as between PC1 and production of the intestinal PGDPs. These findings provide support for the suggestion that PC1 and PC2 play roles in the tissue-specific post-translational processing of proglucagon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:55:45