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Titolo:
OXIDATION OF LOW-DENSITY-LIPOPROTEIN BY HUMAN MONOCYTE-MACROPHAGES RESULTS IN TOXICITY TO THE OXIDIZING CULTURE
Autore:
MARCHANT CE; VANDERVEEN C; LAW NS; HARDWICK SJ; CARPENTER KLH; MITCHINSON MJ;
Indirizzi:
UNIV CAMBRIDGE,DEPT PATHOL,DIV CELLULAR PATHOL,TENNIS COURT RD CAMBRIDGE CB2 1QP ENGLAND
Titolo Testata:
Free radical research
fascicolo: 5, volume: 24, anno: 1996,
pagine: 333 - 342
SICI:
1071-5762(1996)24:5<333:OOLBHM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPID-PEROXIDATION; ATHEROSCLEROTIC LESIONS; CHOLESTERYL LINOLEATE; ALPHA-TOCOPHEROL; PROBUCOL; ANTIOXIDANTS; HEPATOCYTES; INCREASES; INVITRO; CELLS;
Keywords:
LOW DENSITY LIPOPROTEIN; OXIDATION; MONOCYTE-MACROPHAGES (HUMAN); CYTOTOXICITY, ANTIOXIDANTS; ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
C.E. Marchant et al., "OXIDATION OF LOW-DENSITY-LIPOPROTEIN BY HUMAN MONOCYTE-MACROPHAGES RESULTS IN TOXICITY TO THE OXIDIZING CULTURE", Free radical research, 24(5), 1996, pp. 333-342

Abstract

Human monocyte-macrophage cultures were exposed to native low densitylipoprotein (LDL) for up to 24 h in Ham's F10 medium and the extent of cell-mediated LDL oxidation was determined by measurement of electrophoretic mobility on agarose gels and measurement of lipids and oxidised lipids (including 7 beta-hydroxycholesterol) by GC. After an initial lag phase, which varied from 2-8 h, there was a steady increase in oxidation over 24 h. No-cell control incubations showed minimal increases in oxidation over 24 h. Significant toxicity, measured as release of radioactivity from macrophages pre-loaded with tritiated adenine, was observed in the cells when they oxidised LDL and the extent of radioactivity release correlated closely with the extent of LDL oxidation. Inhibition of oxidation using alpha-tocopherol or probucol reduced toxicity within the oxidising culture. This self-inflicted toxicity may help to explain the origin and enlargement of the lipid core of advanced atherosclerotic lesions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 23:53:00