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Titolo:
A MONOCLONAL-ANTIBODY REACTIVE WITH A COMMON EPITOPE OF MORAXELLA (BRANHAMELLA) CATARRHALIS LIPOPOLYSACCHARIDES
Autore:
OISHI K; TANAKA H; SONODA F; BORANN S; AHMED K; UTSUNOMIYA Y; WATANABE K; NAGATAKE T; VANEECHOUTTE M; VERSCHRAEGEN G; MATSUMOTO K;
Indirizzi:
NAGASAKI UNIV,INST TROP MED,DEPT INTERNAL MED,1-12-4 SAKAMOTO MACHI NAGASAKI 852 JAPAN STATE UNIV GHENT HOSP,LAB BACTERIOL & VIROL B-9000 GHENT BELGIUM
Titolo Testata:
Clinical and diagnostic laboratory immunology
fascicolo: 3, volume: 3, anno: 1996,
pagine: 351 - 354
SICI:
1071-412X(1996)3:3<351:AMRWAC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
OTITIS-MEDIA; STRAINS; ANTIGENS; CHILDREN; INFECTION; SURFACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
K. Oishi et al., "A MONOCLONAL-ANTIBODY REACTIVE WITH A COMMON EPITOPE OF MORAXELLA (BRANHAMELLA) CATARRHALIS LIPOPOLYSACCHARIDES", Clinical and diagnostic laboratory immunology, 3(3), 1996, pp. 351-354

Abstract

A hybrid cell line producing a monoclonal antibody (MAb) against Moraxella (Branhamella) catarrhalis lipopolysaccharide (LPS) was established. The specificity of the MAb 1B12 to purified rough LPSs from six strains of M. catarrhalis was ascertained by enzyme-linked immunosorbentassay (ELISA), competitive-inhibition ELISA, and immunoblotting. MAb 1B12 bound to live bacterial cells and culture supernatants from a total of 34 strains of M. catarrhalis, including 12 strains with different LPS serotypes. No cross-reactions with smooth and rough LPSs from selected enterobacterial and nonenterobacterial strains, with other respiratory pathogens, or with Neisseria species were observed. These datasuggest that MAb 1B12 recognizes a common epitope of M. catarrhalis LPS which differs from serotype determinants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 19:19:47