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Titolo:
OXIDATIVE STRESS IS INVOLVED IN HEAT-INDUCED CELL-DEATH IN SACCHAROMYCES-CEREVISIAE
Autore:
DAVIDSON JF; WHYTE B; BISSINGER PH; SCHIESTL RH;
Indirizzi:
RUAKURA AGR CTR,DEPT BIOL MOLEC,AGRES,E ST,PRIVATE BAG 3123 HAMILTON NEW ZEALAND HARVARD UNIV,SCH PUBL HLTH,DEPT MOLEC & CELLULAR TOXICOL BOSTON MA 02115
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 10, volume: 93, anno: 1996,
pagine: 5116 - 5121
SICI:
0027-8424(1996)93:10<5116:OSIIIH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-EFFICIENCY TRANSFORMATION; INTACT YEAST-CELLS; SUPEROXIDE-DISMUTASE; SHOCK RESPONSE; CATALASE-T; THERMOTOLERANCE; INDUCTION; PROTEIN; GENE; TOXICITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.F. Davidson et al., "OXIDATIVE STRESS IS INVOLVED IN HEAT-INDUCED CELL-DEATH IN SACCHAROMYCES-CEREVISIAE", Proceedings of the National Academy of Sciences of the United Statesof America, 93(10), 1996, pp. 5116-5121

Abstract

The cause for death after lethal heat shock is not well understood. Ashift from low to intermediate temperature causes the induction of heat-shock proteins in most organisms. However, except for HSP104, a convincing involvement of heat-shock proteins in the development of stress resistance has not been established in Saccharomyces cerevisiae. This paper shows that oxidative stress and antioxidant enzymes play a major role in heat-induced cell death in yeast. Mutants deleted for the antioxidant genes catalase, superoxide dismutase, and cytochrome c peroxidase were more sensitive to the lethal effect of heat than isogenic wild-type cells. Overexpression of catalase and superoxide dismutase genes caused an increase in thermotolerance. Anaerobic conditions caused a 500- to 20,000-fold increase in thermotolerance. The thermotolerance of cells in anaerobic conditions was immediately abolished upon oxygen exposure. HSP104 is not responsible for the increased resistance of anaerobically grown cells. The thermotolerance of anaerobically grown cells is not due to expression of heat-shock proteins. By using an oxidation-dependent fluorescent molecular probe a 2- to 3-fold increasein fluorescence was found upon heating. Thus, we conclude that oxidative stress is involved in heat-induced cell death.

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Documento generato il 02/12/20 alle ore 14:22:34