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Titolo:
EVIDENCE THAT CCK-58 HAS STRUCTURE THAT INFLUENCES ITS BIOLOGICAL-ACTIVITY
Autore:
REEVE JR; EYSSELEIN VE; ROSENQUIST G; ZEEH J; REGNER U; HO FJ; CHEW P; DAVIS MT; LEE TD; SHIVELY JE; BRAZER SR; LIDDLE RA;
Indirizzi:
DEPT VET AFFAIRS,CURE,DIGEST DIS RES CTR,BLDG 115,RM 115 LOS ANGELES CA 90073 UNIV CALIF LOS ANGELES,SCH MED,DIV DIGEST DIS LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,HARBOR MED CTR,DIV GASTROENTEROL TORRANCE CA 90509 UNIV CALIF DAVIS,DIV ANIM PHYSIOL DAVIS CA 95616 CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL DUARTE CA 91010 UNIV ESSEN GESAMTHSCH,DIV GASTROENTEROL D-4300 ESSEN GERMANY UNIV MANNHEIM,DEPT MED,DIV GASTROENTEROL D-6800 MANNHEIM GERMANY DUKE UNIV,DEPT MED DURHAM NC 27710 DUKE VET AFFAIRS MED CTR DURHAM NC 27710
Titolo Testata:
American journal of physiology: Gastrointestinal and liver physiology
fascicolo: 5, volume: 33, anno: 1996,
pagine: 860 - 868
SICI:
0193-1857(1996)33:5<860:ETCHST>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANINE INTESTINAL CHOLECYSTOKININ-58; PANCREATIC ACINI; STIMULATION; PLASMA; BRAIN; FORM;
Keywords:
MOLECULAR FORMS; AMYLASE; PANCREATIC ACINI; BIOASSAY; RADIOIMMUNOASSAY; GASTROINTESTINAL HORMONE; DOG; CHOLECYSTOKININ-58; CONFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
J.R. Reeve et al., "EVIDENCE THAT CCK-58 HAS STRUCTURE THAT INFLUENCES ITS BIOLOGICAL-ACTIVITY", American journal of physiology: Gastrointestinal and liver physiology, 33(5), 1996, pp. 860-868

Abstract

Many biologically active peptides exist in multiple molecular forms, but the functional significance of regions outside the region of bioactivity is unknown. The biological and immunological data presented in this study indicate that cholecystokinin-58 (CCK-58), unlike other forms of cholecystokinin, has structure that influences its bioactivity. CCK-58 was purified from acid extracts of canine intestinal mucosa until a single absorbance peak was obtained during reverse-phase chromatography Amino acid analysis precisely determined the peptide concentrations of purified CCK-58 and synthetic CCK-8. Our hypothesis was that if the amino terminus of CCK-58 influences its bioactivity then its activity would be modified when this region was removed from the peptide. To evaluate the importance of the amino terminus of CCK-58 to influence its biological activity, the abilities of CCK-58 and CCK-8 to release amylase from pancreatic acini were compared before and after tryptic digestion. Tryptic digestion of CCK-58 decreased the half-maximal stimulation (EC(50)) for amylase release from 96 to 28 pM. The EC(50) for digested CCK-58 was similar to that for CCK-8 (17 pM). These resultssuggest that CCK-58 has a structure that shields its bioactive carboxyl terminus. This is further supported by the finding that carboxyl fragments generated from CCK-58 by trypsin or by partial acid hydrolysiswere greater than twofold more immunoreactive than the intact CCK-58. The diminished activity of CCK-58 indicates that the amino terminus of CCK-58 shields the carboxyl terminus, which is important to its biological and immunological activities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 20:18:17