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Titolo:
TYPING OF VEROTOXINS BY DNA COLONY HYBRIDIZATION WITH POLYNUCLEOTIDE AND OLIGONUCLEOTIDE PROBES, A BEAD-ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND POLYMERASE CHAIN-REACTION
Autore:
YAMASAKI S; LIN Z; SHIRAI H; TERAI A; OKU Y; ITO H; OHMURA M; KARASAWA T; TSUKAMOTO T; KURAZONO H; TAKEDA Y;
Indirizzi:
INT MED CTR JAPAN,RES INST,SHINJUKU KU,1-21-1 TOYAMA TOKYO 158 JAPAN INT MED CTR JAPAN,RES INST,SHINJUKU KU TOKYO 158 JAPAN KYOTO UNIV,FAC MED,DEPT MICROBIOL,SAKYO KU KYOTO 60601 JAPAN NISSUI PHARMACEUT CO,INST DIAGNOST REAGENTS YUKI IBARAKI 307 JAPAN OSAKA PREFECTURAL INST PUBL HLTH,HIGASHI KU OSAKA 537 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 5, volume: 40, anno: 1996,
pagine: 345 - 352
SICI:
0385-5600(1996)40:5<345:TOVBDC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHIGA-LIKE-TOXIN; ESCHERICHIA-COLI O157-H7; NUCLEOTIDE-SEQUENCE; EDEMA DISEASE; VERO TOXIN; DISTINCT TOXINS; VARIANT GENES; CLONING; PURIFICATION; STRAIN;
Keywords:
VEROTOXIN; SHIGA-LIKE TOXIN; TYPING; COLONY HYBRIDIZATION TEST; PCR; BEAD-ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
S. Yamasaki et al., "TYPING OF VEROTOXINS BY DNA COLONY HYBRIDIZATION WITH POLYNUCLEOTIDE AND OLIGONUCLEOTIDE PROBES, A BEAD-ENZYME-LINKED IMMUNOSORBENT-ASSAY, AND POLYMERASE CHAIN-REACTION", Microbiology and immunology, 40(5), 1996, pp. 345-352

Abstract

To identify the type of Verotoxins (VT) produced by Verocytotoxin-producing Escherichia coli (VTEC), a sensitive bead-enzyme-linked immunosorbent assay and polymerase chain reaction with common and specific primers to various VTs (VT1, VT2, VT2vha, VT2vhb, and VT2vp1) were developed. Together with colony hybridization tests with oligo- and polynucleotide probes, these methods were applied to VTEC isolates to type the VT produced. The toxin types of 26 of 37 strains were identified, but the reaction profiles in assays of the remaining 11 strains suggested the existence of new VT2 variants. The application of these identification procedures may be useful as a tool for clinical and epidemiological studies of VTEC infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:09:32