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Titolo:
RELATIONSHIP OF HIV-1 ENVELOPE V2 AND V3 SEQUENCES OF THE PRIMARY ISOLATES TO THE VIRAL PHENOTYPE
Autore:
YOSHIMURA K; MATSUSHITA S; HAYASHI A; TAKATSUKI K;
Indirizzi:
KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 2,1-1-1 HONJO KUMAMOTO 860 JAPAN KUMAMOTO UNIV,SCH MED,DEPT INTERNAL MED 2 KUMAMOTO 860 JAPAN KUMAMOTO UNIV,SCH MED,DEPT TRANSFUS SERV KUMAMOTO 860 JAPAN
Titolo Testata:
Microbiology and immunology
fascicolo: 4, volume: 40, anno: 1996,
pagine: 277 - 287
SICI:
0385-5600(1996)40:4<277:ROHEVA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; AMINO-ACID CHANGES; T-CELL-LINE; SYNCYTIUM FORMATION; MACROPHAGE TROPISM; VARIABLE REGIONS; DOMAIN; GP120; PROTEIN; LOOP;
Keywords:
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1); PRIMARY ISOLATES; V2 AND V3 SEQUENCES; VIRAL PHENOTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
K. Yoshimura et al., "RELATIONSHIP OF HIV-1 ENVELOPE V2 AND V3 SEQUENCES OF THE PRIMARY ISOLATES TO THE VIRAL PHENOTYPE", Microbiology and immunology, 40(4), 1996, pp. 277-287

Abstract

We examined the relationship between the amino acid sequences of the V2 and V3 regions of the envelope protein and the biological properties of ten human immunodeficiency virus type 1 (HIV-1) primary isolates. The infectivity, cytopathic effect (CPE), and syncytium forming activity of these primary isolates were tested against three T cell lines (CEM, MT2, and MOLT4/CL.8 cells), CD8-depleted peripheral blood mononuclear cells (PBMC), and primary monocyte-derived macrophages (MDM) fromseronegative donors. In addition to the viral groups which had the syncytium inducing/T-cell line tropic (SI/TT) phenotype or non-syncytiuminducing/non-T cell line tropic (NSI/NT) phenotype (including the NSI/macrophage tropic (NSI/MT) phenotype), there was a group of viruses that infected one or two T cell lines and PBMC but could not mediate syncytium formation. We therefore classified this group of viruses as a non-syncytium inducing/partial T-cell line tropic (NSI/pTT) virus. To investigate the relationship between these viral phenotypes and the sequence variability of the V2 and V3 regions of the envelope, we clonedthe viral gene segment and sequenced the individual isolates, The sequence data suggested that the SI/TT type changes in the V3 sequence alone mediate a partial T cell line tropism and mild cytopathic effect and that an isolate became more virulent (SI/TT phenotype) if there were additional changes in the V2 or other regions. On the other hand, sequence changes in the V2 region alone could not mediate phenotypic changes but some additional changes in the other variable regions (for example, V3) might be required for the phenotypic changes in combinationwith changes in V2. These findings also suggested that amino acid changes in both the V2 and V3 region are required for the development of virulent variants of HIV-1 that outgrow during advanced stages of the disease.

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Documento generato il 04/12/20 alle ore 05:54:07