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Titolo:
OCTREOTIDE DIFFERENTIALLY MODULATES HUMAN CACO-2 INTESTINAL EPITHELIAL-CELL PROLIFERATION AND DIFFERENTIATION BY DECREASING INTRACELLULAR CAMP
Autore:
SGAMBATI SA; ZARIF A; BASSON MD;
Indirizzi:
YALE UNIV,SCH MED,DEPT SURG,333 CEDAR ST,BOX 208062 NEW HAVEN CT 06520 YALE UNIV,SCH MED,DEPT SURG NEW HAVEN CT 06520 UNIV CONNECTICUT,SCH MED,DEPT SURG NEW HAVEN CT 06520 W HAVEN VET AFFAIRS MED CTR,DEPT SURG NEW HAVEN CT 06520
Titolo Testata:
Regulatory peptides
fascicolo: 3, volume: 61, anno: 1996,
pagine: 219 - 227
SICI:
0167-0115(1996)61:3<219:ODMHCI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; SOMATOSTATIN ANALOGS; ENTEROCYTIC DIFFERENTIATION; PANCREATIC-CANCER; DNA-SYNTHESIS; INVITRO; LINE; MIGRATION; PROTEINS; REGENERATION;
Keywords:
ADENYLATE CYCLASE; ALKALINE PHOSPHATASE; CELL-MATRIX INTERACTION; CELLULAR MOTILITY; DIPEPTIDYL DIPEPTIDASE; SOMATOSTATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Sgambati et al., "OCTREOTIDE DIFFERENTIALLY MODULATES HUMAN CACO-2 INTESTINAL EPITHELIAL-CELL PROLIFERATION AND DIFFERENTIATION BY DECREASING INTRACELLULAR CAMP", Regulatory peptides, 61(3), 1996, pp. 219-227

Abstract

Somatostatin modulates gastrointestinal mucosal growth and differentiation indirectly via inhibition of bioactive peptides and directly by less well understood mechanisms. We studied the direct effects of the somatostatin analog octreotide on proliferation, brush-border enzyme activity, cell-matrix interactions and intracellular cAMP in Caco-2 human intestinal epithelial cells. Proliferation was assessed by cell counting and [H-3]thymidine uptake. The brush-border enzymes alkaline phosphatase (AP) and dipeptidyl dipeptidase (DP) were quantitated by synthetic substrate digestion. Adhesion and migration on purified matrix proteins were also measured. Octreotide (10(-9)-10(-5) M) shortened doubling time (46.5 +/- 6.2% at 10(-5) M, n = 20, P < 0.0001) and stimulated [H-3]thymidine uptake. Octreotide decreased intracellular cAMP by 19.4 +/- 5.0% (n = 7, P < 0.0001) while dibutyryl-cAMP (10(-6) M) prolonged doubling time by 10.1 +/- 1.5% (n = 8, P < 0.0001), and blocked the octreotide effect. Octreotide decreased AP and DP with maximal effect at 10(-6) M (36.8 +/- 8.3% and 20.5 +/- 9.1%, n > 7, P < 0.0005 respectively). However, mitomycin proliferative blockade prevented octreotide inhibition of AP and DP, suggesting that the mitogenic effects of octreotide had simply decreased average maturity of the cells. Octreotide did not alter Caco-2 adhesion, EGF-or matrix-modulated motility,or integrin surface expression. Octreotide appears to directly stimulate Caco-2 proliferation by decreasing cAMP. These proliferative effects modulate Caco-2 differentiation but do not affect cell-matrix interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 01:28:34