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Titolo:
EXPRESSION OF RAB3D IN DISPERSED CHIEF CELLS FROM GUINEA-PIG STOMACH
Autore:
RAFFANIELLO RD; LIN JY; WANG FS; RAUFMAN JP;
Indirizzi:
SUNY HLTH SCI CTR,DEPT MED,GASTROINTESTINAL CELL BIOL LAB,450 CLARKSON AVE BROOKLYN NY 11203 SUNY HLTH SCI CTR,DEPT MED,GASTROINTESTINAL CELL BIOL LAB BROOKLYN NY11203
Titolo Testata:
Biochimica et biophysica acta. Molecular cell research
fascicolo: 2, volume: 1311, anno: 1996,
pagine: 111 - 116
SICI:
0167-4889(1996)1311:2<111:EORIDC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEINS; PEPSINOGEN SECRETION; SYNAPTIC VESICLES; EXOCYTOSIS; GTPASES; CLONING; FAMILY; BRAIN; GENE;
Keywords:
SIGNAL TRANSDUCTION; RAS PROTEIN; LOW MOLECULAR WEIGHT GTP-BINDING PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Raffaniello et al., "EXPRESSION OF RAB3D IN DISPERSED CHIEF CELLS FROM GUINEA-PIG STOMACH", Biochimica et biophysica acta. Molecular cell research, 1311(2), 1996, pp. 111-116

Abstract

Rab3 proteins are low molecular weight GTP-binding proteins that are expressed in neurons and other secretory cells. These proteins are localized to secretory vesicles and may play a role in regulated exocytosis. Presently, four highly homologous Rab3 isoforms (A, B, C, D) have been identified. We examined the expression of Rab3 isoforms in dispersed chief cells from guinea pig stomach. Immunoblotting with a specific monoclonal Rab3 antibody detected a 27-kDa protein in chief cell cytosolic and membrane fractions, but staining was more intense in membrane fractions. Using the Rab3 antibody, immunohistochemical staining was detected in chief cells but not in parietal or mucous cells. To determine which Rab3 isoform(s) is (are) expressed, chief cell cDNA was obtained by reverse transcription and subjected to PCR using degenerate primers that are specific for rab3 isoforms. The resulting PCR products were cloned and sequenced. The nucleotide sequences obtained were 89% homologous to the nucleotide sequence of mouse rab3D. The deduced amino acid sequence was identical to that of mouse Rab3D (amino acids 16-83). Moreover, Rab3D was the only isoform detected in chief cells by these methods. To identify rab3 transcripts, the guinea pig rab3D fragment obtained by reverse transcription PCR cloning was used as a probefor Northern blotting. The 4.0- and 2.3-kb transcripts identified in chief cells with the rab3 probe were the same size as those detected by others in mouse adipocytes using a rab3D-specific probe. These results indicate that Rab3D is expressed in gastric chief cells.

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Documento generato il 14/07/20 alle ore 03:11:43