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Titolo:
SERUM ALPHA-TOCOPHEROL, VITAMIN-A, AND BLOOD SELENIUM CONCENTRATIONS,AND GLUTATHIONE-PEROXIDASE ACTIVITY IN LLAMAS FED ALFALFA HAY
Autore:
DART AJ; KINDE H; HODGSON DR; PEAUROI JR; SELBY AW; MAAS J; FOWLER ME;
Indirizzi:
UNIV SYDNEY,SPECIALIST VET CTR CAMDEN NSW 2570 AUSTRALIA UNIV CALIF DAVIS,CALIF DIAGNOST LAB SYST DAVIS CA 95616 UNIV CALIF DAVIS,DEPT VET MED DAVIS CA 95616
Titolo Testata:
American journal of veterinary research
fascicolo: 5, volume: 57, anno: 1996,
pagine: 689 - 692
SICI:
0002-9645(1996)57:5<689:SAVABS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATTLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Dart et al., "SERUM ALPHA-TOCOPHEROL, VITAMIN-A, AND BLOOD SELENIUM CONCENTRATIONS,AND GLUTATHIONE-PEROXIDASE ACTIVITY IN LLAMAS FED ALFALFA HAY", American journal of veterinary research, 57(5), 1996, pp. 689-692

Abstract

Objectives-To measure blood selenium concentration and glutathione peroxidase (GSH-Px) activity and serum concentrations of vitamin A and alpha-tocopherol, and to determine the correlation between blood selenium concentration and GSH-Px activity of llamas fed alfalfa hay. Design-Mean (+/- SD) serum vitamin A and alpha-tocopherol concentrations, blood selenium concentrations, and GSH-Px activity were calculated from 9 sequential blood samples collected from llamas fed a diet of alfalfahay. Animals-15 clinically normal llamas (8 males, 7 females) between10 and 14 months of age. Procedure-Llamas were fed alfalfa hay for 40days prior to sample collection and then for the duration of the trial. Vitamin E, selenium, and concentrations of vitamin A precursors were measured in the hay. Blood samples were collected on days 0, 6, 7, 9, 13, 20, 42, 64, and 98. Blood selenium concentrations were measured,using an inductively coupled spectrometric method. Blood GSH-Px activity was measured with a spectrophotometer, using a modification of a previously described assay. Isocratic high-performance liquid chromatography with florescent detection was used to determine serum alpha-tocopherol and vitamin A concentrations. Results-The alfalfa hay contained0.2 mg/kg of selenium, 5 mg/kg of vitamin E, and 0.9 mg/kg of vitaminA precursors. The mean ia SD) blood selenium concentration and GSH-Pxactivity were 0.179 +/- 0.032 mu g/ml and 25.76 +/- 6.53 mU NADPH oxidized/min/mg of Hb, respectively, with a correlation coefficient of 0.97. The mean (+/- SD) concentrations for serum a-tocopherol and vitamin A were 128.1 +/- 41.7 and 74.8 +/- 5.5 mu g/dl, respectively. Conclusions-Blood selenium concentrations in llamas are highly correlated toGSH-Px activity. Blood selenium concentrations in llamas appear to besimilar to other domestic ruminants and diets containing 0.2 mg/kg ofselenium appear to provide an adequate dietary source. The concentrations of vitamin A precursors and vitamin E in the hay were below currently recommended dietary levels for llamas, and alfalfa hay appears toprovide an unreliable source of vitamins A and E in this species. Further studies are required to determine optimal dietary concentrations and to substantiate a reference range for these vitamins in llamas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 12:13:41