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Titolo:
SELECTIVE HIPPOCAMPAL-LESIONS DIFFERENTIALLY AFFECT THE PHENOTYPIC FATE OF TRANSPLANTED NEURONAL PRECURSOR CELLS
Autore:
SHIHABUDDIN LS; HOLETS VR; WHITTEMORE SR;
Indirizzi:
UNIV MIAMI,SCH MED,MIAMI PROJECT CURE PARALYSIS,PROGRAM NEUROSCI MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT NEUROL SURG MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT CELL BIOL & ANAT MIAMI FL 33136 UNIV MIAMI,SCH MED,DEPT PHYSIOL & BIOPHYS MIAMI FL 33136
Titolo Testata:
Experimental neurology
fascicolo: 1, volume: 139, anno: 1996,
pagine: 61 - 72
SICI:
0014-4886(1996)139:1<61:SHDATP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; MESENCEPHALIC RAPHE NEURONS; INTRACEREBRAL NEURAL IMPLANTS; STRIATAL GRAFTS; MORPHOLOGICAL DEVELOPMENT; TRANSMITTER EXPRESSION; EMBRYONIC MEDULLARY; TYROSINE-HYDROXYLASE; NEOCORTICAL NEURONS; EXCITOTOXIC LESION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
L.S. Shihabuddin et al., "SELECTIVE HIPPOCAMPAL-LESIONS DIFFERENTIALLY AFFECT THE PHENOTYPIC FATE OF TRANSPLANTED NEURONAL PRECURSOR CELLS", Experimental neurology, 139(1), 1996, pp. 61-72

Abstract

RN33B cells, a CNS-derived neuronal precursor cell line, transplantedinto normal adult rat hippocampus can survive and morphologically differentiate with their ultimate morphology dependent on the integrationsite. This study examined the differentiation and structural integration of RN33B cells transplanted into the lesioned adult hippocampus. Pyramidal neurons of the CA1-3 regions or granular neurons in the dentate gyrus were preferentially destroyed by unilateral intraventricular kainic-acid or intradentate colchicine injections, respectively. One week after the lesion, a suspension of undifferentiated beta-galactosidase (beta-gal)-labeled RN33B cells was stereotaxically transplanted into the lesioned or the contralateral hippocampus. After 5-7 weeks, sections of the recipient brains were analyzed by toluidine blue stainingand immunohistochemistry for beta-gal, GFAP, and OX-42, A reactive gliosis was observed on the lesioned side which persisted up to 7 weeks postlesion (the latest time point examined). RN33B cells survived in the lesioned hippocampus and assumed variable morphologies depending onthe hippocampal layer into which they integrated. Only RN33B cells located in intact or partially damaged cell layers or in the unlesioned contralateral hippocampus differentiated with morphologies similar to those of endogenous neurons characteristic of those layers. Cells located in layers completely depleted of endogenous neurons assumed bipolar morphologies or sent out multiple processes with no structural polarity, unlike the neuronal morphologies characteristically seen in intact hippocampal cell layers. These data suggest that the presence of some endogenous neurons and partially conserved cytoarchitectural organization are essential for immortalized neuroepithelial precursor cells to differentiate into region-specific neuronal cell types. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 13:16:02