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Titolo:
EFFECTS OF LANTHANIDE IONS ON THE BINDING ABILITY OF ANNEXIN-V TO PHOSPHOLIPID VESICLE
Autore:
FUNAKOSHI T; FURUSHIMA F; MIZOKAMI H; KOJIMA S;
Indirizzi:
KUMAMOTO UNIV,FAC PHARMACEUT SCI,DEPT HYG CHEM KUMAMOTO 862 JAPAN CHEMOSERO THERAPEUT RES INST KUMAMOTO 860 JAPAN
Titolo Testata:
Biochemistry and molecular biology international
fascicolo: 5, volume: 38, anno: 1996,
pagine: 965 - 972
SICI:
1039-9712(1996)38:5<965:EOLIOT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLACENTAL ANTICOAGULANT PROTEIN; PROTHROMBIN; ACTIVATION;
Keywords:
ANNEXIN V; LANTHANIDE IONS; BINDING ABILITY; PHOSPHOLIPID VESICLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
T. Funakoshi et al., "EFFECTS OF LANTHANIDE IONS ON THE BINDING ABILITY OF ANNEXIN-V TO PHOSPHOLIPID VESICLE", Biochemistry and molecular biology international, 38(5), 1996, pp. 965-972

Abstract

The effects of lanthanide ions (Ln(3+)), La3+, Ce3+, Gd3+, and Tb3+, on the binding ability of annexin V to phospholipid vesicle were studied, and compared with that of Ca2+. The energy transfer following excitation at 278 nm was accompanied by a small decrease (about 3%) in thefluorescence signal (310-315 nm) of the aromatic amino acid residues,followed by strong re-emission of annexin V-bound Tb3+ at 547 nm. Theresults of the titration of Tb3+-protein interactions using the rate dialysis method suggest that there are two high affinity metal bindingsites on annexin V, to which Tb3+ binds with an average dissociation constant, Kd, of about 2.11 x 10(-7) M. The Ln(3+) seemed to assist the anticoagulant action of annexin V because it binds more strongly to phospholipid than Ca2+ does. Annexin V had the same ability to bind phospholipids in 10 mu M Ln(3+) as it did in 1 mM Ca2+, showing that it binds to anionic phospholipids with Ln(3+) more strongly than it does with Ca2+.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:32:14