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Titolo:
RESERPINE-SENSITIVE AND TETRABENAZINE-SENSITIVE TRANSPORT OF H-3 HISTAMINE BY THE NEURONAL ISOFORM OF THE VESICULAR MONOAMINE TRANSPORTER
Autore:
ERICKSON JD; EIDEN LE; SCHAFER MKH; WEIHE E;
Indirizzi:
NIMH,MOLEC NEUROSCI SECT,CELL BIOL LAB,NIH BETHESDA MD 20892 UNIV MARBURG,DEPT ANAT & CELL BIOL W-3550 MARBURG GERMANY
Titolo Testata:
Journal of molecular neuroscience
fascicolo: 4, volume: 6, anno: 1995,
pagine: 277 - 287
SICI:
0895-8696(1995)6:4<277:RATTOH>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; HISTAMINE SYNTHESIS; MOUSE-BRAIN; ALPHA-FLUOROMETHYLHISTIDINE; IN-VIVO; RELEASE; CELLS; HYPOTHALAMUS; SLICES; 5-HYDROXYTRYPTAMINE;
Keywords:
VESICULAR MONOAMINE TRANSPORTER ISOFORMS; VMAT1; VMAT2; HISTAMINE; RESERPINE; TETRABENAZINE; POSTERIOR HYPOTHALAMUS; TUBEROMAMMILLARY NUCLEUS; MAST CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Erickson et al., "RESERPINE-SENSITIVE AND TETRABENAZINE-SENSITIVE TRANSPORT OF H-3 HISTAMINE BY THE NEURONAL ISOFORM OF THE VESICULAR MONOAMINE TRANSPORTER", Journal of molecular neuroscience, 6(4), 1995, pp. 277-287

Abstract

The transport of H-3-histamine by the endocrine-specific (VMAT1) and neuronal (VMAT2) isoforms of the vesicular monoamine transporter has been evaluated in digitonin-permeabilized fibroblasts transfected with either VMAT1 or VMAT2. Transport of H-3-histamine by both VMAT1 and VMAT2 was reserpine-sensitive but only transport by VMAT2 was inhibited by tetrabenazine. Maximal equilibrated levels of H-3-histamine accumulation by VMAT2 (K-m similar to 300 mu M) were approximately three times greater than that mediated by VMAT1 when using a subsaturating concentration of exogenous H-3-histamine (50 mu M). The expression of VMAT2in histaminergic neurons in the rat brain was examined with polyclonal antipeptide antibodies specific for VMAT1 or VMAT2. VMAT2-positive and tyrosine hydroxylase-negative immunoreactive cell bodies were localized to the ventral part of the posterior hypothalamus in the region of the mamillary nuclei. The transport properties of VMAT2 and the distribution of VMAT2 in cell bodies in the tuberomammillary nucleus of the posterior hypothalamus reported here and the apparent absence of VMAT1 and VMAT2 in tissue mast cells support previous findings of reserpine-sensitive and reserpine-resistant pools of histamine in brain and peripheral tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:22:10