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Titolo:
INTERLEUKIN-12 IS PRODUCED BY DENDRITIC CELLS AND MEDIATES T-HELPER 1DEVELOPMENT AS WELL AS INTERFERON-GAMMA PRODUCTION BY T-HELPER-1 CELLS
Autore:
HEUFLER C; KOCH F; STANZL U; TOPAR G; WYSOCKA M; TRINCHIERI G; ENK A; STEINMAN RM; ROMANI N; SCHULER G;
Indirizzi:
INNSBRUCK UNIV,DEPT DERMATOL,ANICHSTR 35 A-6020 INNSBRUCK AUSTRIA WISTAR INST ANAT & BIOL PHILADELPHIA PA 00000 UNIV MAINZ,DEPT DERMATOL MAINZ GERMANY ROCKEFELLER UNIV,CELLULAR PHYSIOL & IMMUNOL LAB NEW YORK NY 10021
Titolo Testata:
European Journal of Immunology
fascicolo: 3, volume: 26, anno: 1996,
pagine: 659 - 668
SICI:
0014-2980(1996)26:3<659:IIPBDC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; COLONY-STIMULATING FACTOR; ACCESSORY CELLS; LYMPHOCYTES-T; IMMUNITY; REQUIREMENTS; CYTOKINE; CULTURE; IL-12; MICE;
Keywords:
INTERLEUKIN-12; DENDRITIC CELLS; TH1; TH2; LANGERHANS CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C. Heufler et al., "INTERLEUKIN-12 IS PRODUCED BY DENDRITIC CELLS AND MEDIATES T-HELPER 1DEVELOPMENT AS WELL AS INTERFERON-GAMMA PRODUCTION BY T-HELPER-1 CELLS", European Journal of Immunology, 26(3), 1996, pp. 659-668

Abstract

Interleukin-12 (IL-12), a 70-kDa heterodimeric cytokine composed of covalently linked p35 and p40 chains, is to date the most critical factor for skewing the immune response towards a T helper 1 (Th 1) of cytokine profile [high interferon-gamma (IFN-gamma), low IL-4]. Established sources of IL-12 are stimulated macrophages, neutrophils and B cells. As dendritic cells (DC) process antigen in the periphery and then migrate to lymphoid organs to sensitize T cells and induce cell-mediatedimmunity, we reasoned that DC should constitute a critical source of IL-12. The criteria used to detect IL-12 in DC were the demonstration of p40 and p35 mRNA (semiquantitative polymerase chain reaction, Northern blotting, and in situ hybridization) as well as IL-12 protein (p70enzyme-linked immunosorbent assay, p70 antigen capture followed by IFN-gamma bioassay, free p40 chain radioimmunoassay or immunoprecipitation). We found that conventional stimuli such as Staphylococcus aureus induced production of IL-12, by murine as well as human DC in amounts comparable to spleen cells. peritoneal macrophages or peripheral bloodmononuclear cells. DC exhibited, however, features that had not been seen with other antigen-presenting cells: they produced bioactive IL-12 upon antigen-specific interaction with T cells without any other stimuli: in an allogeneic mixed leukocyte reaction model. neutralizing anti-IL-12 antibodies showed that DC-derived IL-12 was critical for optimal proliferation and IFN-gamma production by activated Th1 blasts; and finally, the priming of resting, naive allogeneic T cells by DC, followed by restimulation of primed T blasts by DC, skewed the response to Th1 without the need for any exogenous cytokines or stimuli such as microorganisms. This skewing to Th1 cytokine production, which depended on DC-derived IL-12, but did not require anti-IL-4, exogenous IL-12,or microbes, might be a major function of DC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:32:19