Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DIFFERENT CYTOKINES REGULATE ANTIGEN UPTAKE AND PRESENTATION OF A PRECURSOR DENDRITIC CELL-LINE
Autore:
LUTZ MB; ASSMANN CU; GIROLOMONI G; RICCIARDICASTAGNOLI P;
Indirizzi:
UNIV MILAN,CNR,CTR CYTOPHARMACOL,VIA VANVEITELLI 32 I-20129 MILAN ITALY UNIV MILAN,CNR,CTR CYTOPHARMACOL I-20129 MILAN ITALY IRCCS,IST DERMOPATICO IMMACOLATA,IMMUNOL LAB ROME ITALY
Titolo Testata:
European Journal of Immunology
fascicolo: 3, volume: 26, anno: 1996,
pagine: 586 - 594
SICI:
0014-2980(1996)26:3<586:DCRAUA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; COLONY-STIMULATING FACTOR; MOUSE BONE-MARROW; T-CELLS; IN-VIVO; PROTEIN ANTIGENS; PRESENTING CELLS; INVITRO; RESPONSES; IDENTIFICATION;
Keywords:
DENDRITIC CELLS; PINOCYTOSIS; ANTIGEN PRESENTATION; CYTOKINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Lutz et al., "DIFFERENT CYTOKINES REGULATE ANTIGEN UPTAKE AND PRESENTATION OF A PRECURSOR DENDRITIC CELL-LINE", European Journal of Immunology, 26(3), 1996, pp. 586-594

Abstract

Langerhans cells (LC) and dendritic cells (DC) need to he activated in order to perform their antigen-presenting function. In this study, we explored the influence of cytokines on the uptake and presentation of protein antigens by the retrovirally immortalized myeloid cell line FSDC. This cell line was generated from mouse fetal skin and was previously shown to have the characteristics of early DC precursors. Both FSDC and hone marrow-derived DC (BM-DC) were more effective in the pinocytosis of FITC-conjugated ovalbumin (FITC-OVA) and dextran (FITC-DX) than B cells or macrophages. Pretreatment of FSDC with granulocyte/macrophage colony-stimulating factor (GM-CSF) +/- interleukin (IL)-1 enhanced the pinocytic uptake of FITC-OVA, and FITC-DX, but did not induceantigen-presenting capacity. In contrast, untreated FSDC or FSDC pre-incubated with GM-CSF +/- IL-4 suppressed T cell responses. Treatment of FSDC with IFN-gamma reduced pinocytosis but increased the expression of MHC and co-stimulatory/adhesion molecules and promoted efficient presentation of OVA protein or peptide to the specific DO11.10 T cell hybridoma or to naive CD4(+) T cells from DO11.10 TCR-transgenic mice. The results suggest that antigen uptake and antigen presentation in DC are regulated by different cytokine signals provided by the surrounding tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:34:00